TRIM25 Antibody

Images

 
Western Blot: TRIM25 Antibody [H00007706-B01P] - Analysis of TRIM25 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody [H00007706-B01P] - Analysis of purified antibody to TRIM25 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: TRIM25 Antibody [H00007706-B01P] - Analysis of TRIM25 expression in transfected 293T cell line by TRIM25 polyclonal antibody. Lane 1: TRIM25 transfected lysate(69.3 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TRIM25 Antibody Summary

Immunogen
TRIM25 (AAH16924.1, 1 a.a. - 630 a.a.) full-length human protein. MAELCPLAEELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCRAVYQARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQELTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK
Specificity
TRIM25 - tripartite motif-containing 25,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
TRIM25
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRIM25 Antibody

  • EC 6.3.2.19
  • EC 6.3.2.n3
  • EFPZ147
  • Estrogen-responsive finger protein
  • RING finger protein 147
  • RNF147E3 ubiquitin/ISG15 ligase TRIM25
  • tripartite motif containing 25
  • tripartite motif-containing 25
  • Tripartite motif-containing protein 25
  • Ubiquitin/ISG15-conjugating enzyme TRIM25
  • zinc finger protein 147 (estrogen-responsive finger protein)
  • Zinc finger protein 147
  • zinc finger protein-147
  • ZNF147tripartite motif protein TRIM25

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRIM25
Entrez
Uniprot