TRIM25 Antibody (2B12)

Western Blot: TRIM25 Antibody (2B12) [H00007706-M01] - TRIM25 monoclonal antibody (M01), clone 2B12 Analysis of TRIM25 expression in Hela S3 NE.

Western Blot: TRIM25 Antibody (2B12) [H00007706-M01] - Analysis of TRIM25 expression in transfected 293T cell line by TRIM25 monoclonal antibody (M01), clone 2B12.Lane 1: TRIM25 transfected lysate(71 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: TRIM25 Antibody (2B12) [H00007706-M01] - Detection limit for recombinant GST tagged TRIM25 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

TRIM25 Antibody (2B12) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TRIM25 (NP_005073, 211 a.a. ~ 310 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEK
• Specificity: TRIM25 - tripartite motif-containing 25
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM25
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRIM25 Antibody (2B12)

• EC 6.3.2.19
• EC 6.3.2.n3
• EFP
• EFPZ147
• Estrogen-responsive finger protein
• RING finger protein 147
• RNF147
• RNF147E3 ubiquitin/ISG15 ligase TRIM25
• TRIM25
• tripartite motif containing 25
• tripartite motif-containing 25
• Tripartite motif-containing protein 25
• Ubiquitin/ISG15-conjugating enzyme TRIM25
• zinc finger protein 147 (estrogen-responsive finger protein)
• Zinc finger protein 147
• zinc finger protein-147
• ZNF147
• ZNF147tripartite motif protein TRIM25

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM25
• Entrez:
  • 7706(Human)
• OMIM:
  • 600453(Human)
• Uniprot:
  • NP_005073(Human)