TRIF/TICAM1 Antibody (567212) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clone: 567212
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

TRIF/TICAM1 Antibody (567212) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human TRIF/TICAM1
  Met474-Pro618
  Accession # Q8IUC6
• Specificity: Detects human TRIF/TICAM1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRIF (aa 29‑204), rhTRAM, rhTRIM, rhTRIM5, rhTRIM21, or rhTRIM32 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: IgM-specific Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRIF/TICAM1 Antibody (567212) [Alexa Fluor® 350]

• MGC35334
• Proline-rich, vinculin and TIR domain-containing protein B
• PRVTIRBTIR domain-containing adapter protein inducing IFN-beta
• Putative NF-kappa-B-activating protein 502H
• TICAM1
• TICAM-1TIR domain containing adaptor inducing interferon-beta
• TIR domain-containing adapter molecule 1
• TIR domain-containing adaptor molecule 1
• toll-like receptor adaptor molecule 1
• TRIF
• TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta

Background

TRIF (TIR domain-containing adaptor inducing IFN-beta ; also TICAM1) is a 105-110 kDa cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF-3, -4, and -7, as well as NFkB and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712 amino acids (aa) in length. It contains three TRAF6 bonding motifs (aa 84-91, 248-255 and 299-309), one TIR domain (aa 390-460), a Pro-rich region (aa 614-678), and an overlapping RHIM domain (aa 661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23 aa substitution for aa 31-162 accompanied by a Pro substitution for aa 633-660, a second shows a 44 aa substitution for aa 352-712, and a third shows a 38 aa substitution for aa 271-712. Over aa 474-618, human TRIF shares 53% aa identity with mouse TRIF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.