![Western Blot: Trichohyalin Antibody [NBP2-93477] - Analysis of extracts of various cell lines, using Trichohyalin at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize2/Trichohyalin Antibody-Western Blot-NBP2-93477-img0001.jpg "Western Blot: Trichohyalin Antibody [NBP2-93477] - Analysis of extracts of various cell lines, using Trichohyalin at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human TCHH (NP_009044.2). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TCHH |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 253 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TCHH |
![Immunohistochemistry-Paraffin: Filaggrin Antibody [NBP1-87528] - Analysis in human skin and liver tissues. Corresponding Filaggrin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Filaggrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87528-img0017.jpg)
![Immunohistochemistry-Paraffin: Filaggrin Antibody [NBP1-87528] - Staining of human gastrointestinal, liver, skin and testis using Anti-Filaggrin antibody NBP1-87528 (A) shows similar protein distribution across tissues to independent antibody NBP1-87527 (B).](http://images.novusbio.com/images2/Filaggrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87528-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Filaggrin Antibody [NBP1-87528] - Staining of human cell line HaCaT shows localization to vesicles.](http://images.novusbio.com/images2/Filaggrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87528-img0021.jpg)
![Immunohistochemistry-Paraffin: Involucrin Antibody [NBP2-33742] - Staining in human esophagus and stomach tissues. Corresponding IVL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Involucrin Antibody-Immunohistochemistry-Paraffin-NBP2-33742-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Involucrin Antibody [NBP2-33742] - Staining of human cell line HaCaT shows localization to nuclear bodies, cytosol & centrosome. Antibody staining is shown in green.](http://images.novusbio.com/images2/Involucrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33742-img0004.jpg)
![Immunohistochemistry-Paraffin: Involucrin Antibody [NBP2-33742] - Reconstructed human epidermis tissues are embedded in paraffin. Antigen retrieval: with citrate buffer. Antibody at 1:100. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/images2/Involucrin-Antibody-Immunohistochemistry-Paraffin-NBP2-33742-img0016.jpg)
![Western Blot: Lipoprotein Lipase/LPL Antibody (3A10) [NBP2-01395] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipoprotein lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lipoprotein lipase.](http://images.novusbio.com/images2/Lipoprotein-Lipase-LPL-Antibody-(3A10)-Western-Blot-NBP2-01395-img0012.jpg)
![Immunohistochemistry-Paraffin: Lipoprotein Lipase/LPL Antibody (3A10) [NBP2-01395] - Human endometrium tissue using anti-Lipoprotein lipase mouse monoclonal antibody.](http://images.novusbio.com/images2/Lipoprotein-Lipase-LPL-Antibody-(3A10)-Immunohistochemistry-Paraffin-NBP2-01395-img0011.jpg)
![Flow Cytometry: Lipoprotein Lipase/LPL Antibody (3A10) [NBP2-01395] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Lipoprotein lipase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images2/Lipoprotein-Lipase-LPL-Antibody-(3A10)-Flow-Cytometry-NBP2-01395-img0007.jpg)
![Western Blot: Isoleucyl tRNA synthetase Antibody [H00003376-B01P] - Analysis of IARS expression in human placenta.](http://images.novusbio.com/images2/Isoleucyl-tRNA-synthetase-Antibody-Western-Blot-H00003376-B01P-img0004.jpg)
![Western Blot: Isoleucyl tRNA synthetase Antibody [H00003376-B01P] - Analysis of IARS expression in HepG2.](http://images.novusbio.com/images2/Isoleucyl-tRNA-synthetase-Antibody-Western-Blot-H00003376-B01P-img0003.jpg)
![Western Blot: Isoleucyl tRNA synthetase Antibody [H00003376-B01P] - Analysis of IARS expression in transfected 293T cell line by IARS polyclonal antibody. Lane1:IARS transfected lysate(116.05 KDa). Lane2:Non-transfected lysate.](http://images.novusbio.com/images2/Isoleucyl tRNA synthetase Antibody-Western Blot-H00003376-B01P-img0005.jpg)
![Immunohistochemistry: Loricrin Antibody [NBP1-33610] - Prominent differences in the expressions of innate immune and barrier molecules between sebaceous gland poor (SGR) and sebaceous gland rich (SGP) skin regions. Representative images for immunostaining and quantification of epidermal levels of LOR in SGP and SGR skin sections. Images of negative control stainings are shown in the bottom left corner of SGP immunostainings. Size bars=100um. The graphs show the mean +/- SEM of measured protein levels (*p<0.05; **p<0.01; ***p<0.001, as determined by Mann-Whitney U-test). Image collected and cropped by CiteAb from the following publication (frontiersin.org/articles/10.3389/fimmu.2018.00424/full), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Loricrin-Antibody-Immunohistochemistry-NBP1-33610-img0018.jpg)
![Western Blot: Loricrin Antibody [NBP1-33610] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/Loricrin-Antibody-Western-Blot-NBP1-33610-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Loricrin Antibody [NBP1-33610] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/Loricrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33610-img0014.jpg)
![Western Blot: Hornerin Antibody [NBP1-80807] - Hornerin expression in HCC cell lines. Western blot analysis of Hornerin expression levels in a panel of HCC cell lines. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4719-5), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Hornerin-Antibody-Western-Blot-NBP1-80807-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Hornerin Antibody [NBP1-80807] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/Hornerin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80807-img0006.jpg)
![Immunohistochemistry: Hornerin Antibody [NBP1-80807] - Hornerin overpression in human HCC tumor tissues. Immunohistochemistry of Hornerin expression in hepatocellular carcinoma (HCC) tissues. Hornerin expression in the cytoplasm and membrane is scored as negative (a, e), weak (b, f), moderate (c, g), and strong (d, h). Original magnification, x 100 (a-d); x 400 (e-h) Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4719-5), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Hornerin-Antibody-Immunohistochemistry-NBP1-80807-img0011.jpg)
![Western Blot: S100A1 Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.](http://images.novusbio.com/images2/S100A1-Antibody-(4C4.9)-Western-Blot-NBP2-29403-img0001.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100 Ab (4C4.9).](http://images.novusbio.com/images2/S100A1-Antibody-(4C4.9)-Immunohistochemistry-Paraffin-NBP2-29403-img0003.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100 4C4.9 antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining.](http://images.novusbio.com/images2/S100A1-Antibody-(4C4.9)-Immunohistochemistry-Paraffin-NBP2-29403-img0002.jpg)
![Western Blot: S100B Antibody (15F9NB) [NBP2-45267] - Total protein from human brain (1) and mouse brain (2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/S100B-Antibody-(15F9NB)-Western-Blot-NBP2-45267-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/S100B-Antibody-(15F9NB)-Immunocytochemistry-Immunofluorescence-NBP2-45267-img0011.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in human prostate tissue.](http://images.novusbio.com/images2/S100B-Antibody-(15F9NB)-Immunohistochemistry-Paraffin-NBP2-45267-img0014.jpg)
![TNF-alpha [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "TNF-alpha [Unconjugated]")
![Immunocytochemistry/Immunofluorescence: Basalin Antibody [NBP1-80806] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & nuclear membrane.](http://images.novusbio.com/images2/Basalin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80806-img0008.jpg)
![Immunohistochemistry-Paraffin: Basalin Antibody [NBP1-80806] - Staining of human kidney.](http://images.novusbio.com/images2/Basalin-Antibody-Immunohistochemistry-Paraffin-NBP1-80806-img0012.jpg)
![Immunohistochemistry: Basalin Antibody [NBP1-80806] - Staining of hair shows strong cytoplasmic positivity in cells in internal root sheath.](http://images.novusbio.com/images2/Basalin-Antibody-Immunohistochemistry-NBP1-80806-img0009.jpg)
![Western Blot: Plastin L Antibody [NBP2-19860] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/Plastin-L-Antibody-Western-Blot-NBP2-19860-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Plastin Lstained by Plastin L antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/images2/Plastin-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19860-img0007.jpg)
![Immunohistochemistry-Paraffin: Plastin L Antibody [NBP2-19860] - Paraffin-embedded mouse thymus gland. Plastin L antibody diluted at 1:500.](http://images.novusbio.com/images2/Plastin-L-Antibody-Immunohistochemistry-Paraffin-NBP2-19860-img0006.jpg)
![Immunohistochemistry-Paraffin: Pancreatic Lipase Antibody [NBP2-38958] - Staining of human pancreas shows high expression.](http://images.novusbio.com/images2/Pancreatic-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP2-38958-img0008.jpg)
![Immunohistochemistry-Paraffin: Pancreatic Lipase Antibody [NBP2-38958] - Staining of human pancreas shows high expression.](http://images.novusbio.com/images2/Pancreatic-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP2-38958-img0002.jpg)
![Immunohistochemistry-Paraffin: Pancreatic Lipase Antibody [NBP2-38958] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/images2/Pancreatic-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP2-38958-img0003.jpg)
![Western Blot: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution.](http://images.novusbio.com/images2/Cytokeratin-16-Antibody-(SC52-09)-Western-Blot-NBP2-67559-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Cytokeratin-16-Antibody-(SC52-09)-Immunocytochemistry-Immunofluorescence-NBP2-67559-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Cytokeratin-16-Antibody-(SC52-09)-Immunohistochemistry-Paraffin-NBP2-67559-img0007.jpg)
![Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).](http://images.novusbio.com/images2/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/images2/Factor-VIII-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91761-img0005.jpg)
![Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - Staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and the detection was performed using DAB with hematoxylin counterstaining.](http://images.novusbio.com/images2/Factor-VIII-Antibody-Immunohistochemistry-Paraffin-NB100-91761-img0007.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")