TRH-degrading Ectoenzyme/TRHDE Antibody

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Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and embryonic rat brain hippocampal glial. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antigen ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

TRH-degrading Ectoenzyme/TRHDE Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TRH-degrading Ectoenzyme/TRHDE
Arg64-His1025
Accession # Q8K093
Specificity
Detects mouse and rat TRH-degrading Ectoenzyme/TRHDE in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRHDE
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF2985 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRH-degrading Ectoenzyme/TRHDE Antibody

  • EC 3.4.19.6
  • FLJ22381
  • PAP-II
  • PAP-IIthyroliberinase
  • PGPEP2
  • Pyroglutamyl-peptidase II
  • Thyroliberinase
  • thyrotropin-releasing hormone degrading enzyme
  • thyrotropin-releasing hormone-degrading ectoenzyme
  • TRHDE
  • TRH-DE
  • TRHdegrading Ectoenzyme
  • TRH-degrading Ectoenzyme
  • TRH-DEthyrotropin-releasing hormone degrading ectoenzyme
  • TRH-specific aminopeptidase

Background

TRHDE, also known as pyroglutamyl peptidase II and thyroliberinase, is a metalloprotease that specifically removes pyroglutamate from thyrotropin-releasing hormone, a tripeptide of L-pyroglutamyl-L-histidyl-L-prolineamide. TRH functions as a hypothalamic hypophysiotropic neuropeptide and neurotransmitter/neuromodulator within the central nervous system (1). Inhibitors of TRHDE have potential applications as research and therapeutic agents because TRHDE inactivates TRH (2). TRHDE is a type II transmembrane protein and a soluble form is also present in the serum (1). The recombinant mouse TRHDE corresponds to the ectodomain of the enzyme. Its amino acid sequence is 97% and 95% identical to that of rat and human.

  1. Schmitmeier, S. et al. (2002) Eur. J. Biochem. 269:1278.
  2. Kelly, J.A. et al. (2005) Biochem. J. 389:569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRH-degrading Ectoenzyme/TRHDE Antibody (AF2985)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol TRHDE
Uniprot