TRH-degrading Ectoenzyme/TRHDE Antibody

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and embryonic rat brain hippocampal glial . PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRH-degrading Ectoenzyme/TRHDE at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TRH-degrading Ectoenzyme/TRHDE Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TRH-degrading Ectoenzyme/TRHDE
  Arg64-His1025
  Accession # Q8K093
• Specificity: Detects mouse and rat TRH-degrading Ectoenzyme/TRHDE in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRHDE
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRH-degrading Ectoenzyme/TRHDE Antibody

• EC 3.4.19.6
• FLJ22381
• PAP-II
• PAP-IIthyroliberinase
• PGPEP2
• Pyroglutamyl-peptidase II
• Thyroliberinase
• thyrotropin-releasing hormone degrading enzyme
• thyrotropin-releasing hormone-degrading ectoenzyme
• TRHDE
• TRH-DE
• TRHdegrading Ectoenzyme
• TRH-degrading Ectoenzyme
• TRH-DEthyrotropin-releasing hormone degrading ectoenzyme
• TRH-specific aminopeptidase

Background

TRHDE, also known as pyroglutamyl peptidase II and thyroliberinase, is a metalloprotease that specifically removes pyroglutamate from thyrotropin-releasing hormone, a tripeptide of L-pyroglutamyl-L-histidyl-L-prolineamide. TRH functions as a hypothalamic hypophysiotropic neuropeptide and neurotransmitter/neuromodulator within the central nervous system (1). Inhibitors of TRHDE have potential applications as research and therapeutic agents because TRHDE inactivates TRH (2). TRHDE is a type II transmembrane protein and a soluble form is also present in the serum (1). The recombinant mouse TRHDE corresponds to the ectodomain of the enzyme. Its amino acid sequence is 97% and 95% identical to that of rat and human.

1. Schmitmeier, S. et al. (2002) Eur. J. Biochem. 269:1278.
2. Kelly, J.A. et al. (2005) Biochem. J. 389:569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRH-degrading Ectoenzyme/TRHDE Antibody (AF2985)(1)

Publication using AF2985

• Rodriguez-Molina V, Vargas MA, Joseph-Bravo P, Charli JL NMDA receptor up-regulates pyroglutamyl peptidase II activity in the rat hippocampus. Neurosci. Lett., 2008-11-07;449(3):211-4. 2008-11-07 [PMID: 19013213] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: TRHDE
• Uniprot:
  • Q8K093()