TREML2/TLT-2 Antibody (656906)

M1 mouse myeloid leukemia cell line was stained with Rat Anti-Mouse TREML2/TLT‑2 Monoclonal Antibody (Catalog # MAB6707, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 656906
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TREML2/TLT-2 Antibody (656906) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse TREML2/TLT-2
  His25-Ala270
  Accession # Q2LA85
• Specificity: Detects mouse TREML2/TLT-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) TREM-1, -2, -2b, -3, -4, rmTREML1/TLT-1, rmTREML4/TLT-4, or recombinant human (rh) NKp44 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TREML2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREML2/TLT-2 Antibody (656906)

• C6orf76
• dJ238O23.1
• FLJ13693
• MGC149715
• MGC149716
• TLT2
• TLT-2
• TREML2
• trem-like transcript 2 protein
• triggering receptor expressed on myeloid cells-like 2
• triggering receptor expressed on myeloid cells-like protein 2
• UNQ6268/PRO20473

Background

TREML2, also called TLT-2 (Trem-like transcript 2) is a 33 kDa (predicted) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Mouse TLT-2 is synthesized as a 329 amino acid (aa) precursor that contains a 24 aa signal sequence, a 246 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 38 aa cytoplasmic tail. Within the ECD are an Ig-like V-type domain (aa 25-126) and one potential site for N-linked glycosylation. Mature mouse TLT-2 shares 49% aa sequence identity with mature human TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines.  TLT-2 is also expressed constitutively on CD8⁺ T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TREML2
• Uniprot:
  • Q2LA85()