TREML1/TLT-1 Antibody (268515) (MAB24241)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
268515
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

TREML1/TLT-1 Antibody (268515) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREML1/TLT‑1
Gly18-Cys178
Accession # NP_082039
Specificity
Detects mouse TREML1/TLT‑1 in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TREML1 or rhTREML2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
TREML1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Mouse TREML1/TLT-1 Biotinylated Antibody (Catalog number BAF2424)
Standard: Recombinant Mouse TREML1/TLT-1 (Catalog number 2424-TL)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREML1/TLT-1 Antibody (268515)

  • dJ238O23.3
  • GLTL1825
  • PRO3438
  • TLT1
  • TLT-1
  • TLT1MGC119173
  • TREML1
  • trem-like transcript 1 protein
  • triggering receptor expressed on myeloid cells-like 1
  • Triggering receptor expressed on myeloid cells-like protein 1

Background

Triggering receptor expressed on myeloid cells-like protein 1 (TREML1)/TREM-like transcript-1 (TLT‑1) is a 40 kDa type I transmembrane receptor and member of the TREM family of proteins (1). Mouse TREML1/TLT-1 is synthesized as a 317 amino acid (aa) precursor that contains a 20 aa signal sequence, a 155 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 121 aa cytoplasmic domain. The ECD contains an Ig-like V-type domain (aa 21‑122), and the cytoplasmic region contains a proline-rich region (aa 245‑275) and an immunoreceptor tyrosine-based inhibition motif (ITIM) characterized by the residues S/I/V/LXYXXV/L (1). A splicing variant produces a second isoform that has a six aa substitution for aa 173 in isoform 1. Mature mouse TREML1/TLT-1 shares 65% aa sequence identity with mature human TREML1/TLT-1. TREML1/TLT-1 is expressed exclusively in megakaryocytes and platelets where it colocalizes with CD62P in alpha ‑granules in resting platelets and on the cell surface of platelets activated by thrombin (1‑3). The receptor’s expression is up‑regulated dramatically upon platelet activation (2). Antibodies to single‑chain Fv fragments specific for TLT-1 inhibited thrombin-mediated human platelet aggregation suggesting its role in the regulation of aggregation (5). In addition, soluble fragments including the ECD can be released into serum by proteolysis (3). The phosphorylated TREML1/TLT-1 is able to interact with both SHP-1 and SHP-2 through ITIM (1, 4). SHP-2 interaction enhances Fc epsilon RI-mediated calcium signaling in rat basophilic leukemia cells (1). It is hypothesized that TREML1/TLT-1 plays a role in the innate and adaptive immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TREML1
Entrez
Uniprot