TREML1/TLT-1 Antibody (268515) [Alexa Fluor® 700]

• Reactivity: Mu
• Applications: WB, ELISA(Cap), ELISA(Det)
• Clone: 268515
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 700

TREML1/TLT-1 Antibody (268515) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TREML1/TLT‑1
  Gly18-Cys178
  Accession # NP_082039
• Specificity: Detects mouse TREML1/TLT‑1 in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TREML1 or rhTREML2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREML1/TLT-1 Antibody (268515) [Alexa Fluor® 700]

• dJ238O23.3
• GLTL1825
• PRO3438
• TLT1
• TLT-1
• TLT1MGC119173
• TREML1
• trem-like transcript 1 protein
• triggering receptor expressed on myeloid cells-like 1
• Triggering receptor expressed on myeloid cells-like protein 1

Background

Triggering receptor expressed on myeloid cells-like protein 1 (TREML1)/TREM-like transcript-1 (TLT‑1) is a 40 kDa type I transmembrane receptor and member of the TREM family of proteins (1). Mouse TREML1/TLT-1 is synthesized as a 317 amino acid (aa) precursor that contains a 20 aa signal sequence, a 155 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 121 aa cytoplasmic domain. The ECD contains an Ig-like V-type domain (aa 21‑122), and the cytoplasmic region contains a proline-rich region (aa 245‑275) and an immunoreceptor tyrosine-based inhibition motif (ITIM) characterized by the residues S/I/V/LXYXXV/L (1). A splicing variant produces a second isoform that has a six aa substitution for aa 173 in isoform 1. Mature mouse TREML1/TLT-1 shares 65% aa sequence identity with mature human TREML1/TLT-1. TREML1/TLT-1 is expressed exclusively in megakaryocytes and platelets where it colocalizes with CD62P in alpha ‑granules in resting platelets and on the cell surface of platelets activated by thrombin (1‑3). The receptor’s expression is up‑regulated dramatically upon platelet activation (2). Antibodies to single‑chain Fv fragments specific for TLT-1 inhibited thrombin-mediated human platelet aggregation suggesting its role in the regulation of aggregation (5). In addition, soluble fragments including the ECD can be released into serum by proteolysis (3). The phosphorylated TREML1/TLT-1 is able to interact with both SHP-1 and SHP-2 through ITIM (1, 4). SHP-2 interaction enhances Fc epsilon RI-mediated calcium signaling in rat basophilic leukemia cells (1). It is hypothesized that TREML1/TLT-1 plays a role in the innate and adaptive immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.