TREML1/TLT-1 Antibody (268420) [Unconjugated]

Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 PE-conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Rat Anti-Human TREML1/TLT-1 Monoclonal Antibody (Catalog # MAB2394) or (B) Rat IgG2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 268420
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TREML1/TLT-1 Antibody (268420) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TREML1/TLT‑1
  Gln16-Ser160
  Accession # Q86YW5
• Specificity: Detects human TREML1/TLT‑1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse TREML1 or recombinant human TREML2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: TREML1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREML1/TLT-1 Antibody (268420) [Unconjugated]

• dJ238O23.3
• GLTL1825
• PRO3438
• TLT1
• TLT-1
• TLT1MGC119173
• TREML1
• trem-like transcript 1 protein
• triggering receptor expressed on myeloid cells-like 1
• Triggering receptor expressed on myeloid cells-like protein 1

Background

TREML1 is an inhibitory type I transmembrane protein that has two cytoplasmic ITIMs (Immunoreceptor Tyrosine-based Inhibition Motif). A splice variant lacking ITIMs in its cytoplasmic domain also exists. TREML1 is found only in platelets and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TREML1
• Uniprot:
  • Q86YW5()