Trappin-2/Elafin/Skalp Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Trappin-2/Elafin/Skalp Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Trappin-2/Elafin (R&D Systems, Catalog # 1747-PI)
  Ala23-Gln117
  Accession # P19957
• Specificity: Detects human Trappin-2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM‑2, and human Neutrophil Elastase is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PI3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Trappin-2/Elafin Antibody (Catalog # AF1747)
  ELISA Detection: Human Trappin-2/Elafin Biotinylated Antibody (Catalog # BAF1747)
  Standard: Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trappin-2/Elafin/Skalp Antibody [Biotin]

• cementoin
• Elafin
• Elastase-specific inhibitor
• ESI
• ESIWAP four-disulfide core domain protein 14
• Peptidase inhibitor 3
• peptidase inhibitor 3, skin-derived
• PI3
• PI-3
• pre-elafin
• protease inhibitor 3, skin-derived (SKALP)
• Protease inhibitor WAP3
• SKALP
• SKALPELAFIN
• Skin-derived antileukoproteinase
• Trappin2
• Trappin-2
• WAP four-disulfide core domain 14
• WAP3MGC13613
• WFDC14elafin

Background

Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72-117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally.

1. Schalkwijk, J. et al. (1999) Biochem. J. 340:569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Trappin-2/Elafin/Skalp Antibody (BAF1747)(3)

Publications using BAF1747

• J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants Bone Rep, 2020-09-11;13(0):100715. 2020-09-11 [PMID: 32995386] (ELISA Detection, Human)
• Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection Cell 8/1/2020 [PMID: 32645325]
• Guyot N, Butler MW, McNally P, Weldon S, Greene CM, Levine RL, O'Neill SJ, Taggart CC, McElvaney NG Elafin, an Elastase-specific Inhibitor, Is Cleaved by Its Cognate Enzyme Neutrophil Elastase in Sputum from Individuals with Cystic Fibrosis. J. Biol. Chem., 2008-09-17;283(47):32377-85. 2008-09-17 [PMID: 18799464] (ELISA Development, Western Blot, Human)

Bioinformatics

• Gene Symbol: PI3
• Uniprot:
  • P19957()