TRAP220/MED1 Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

TRAP220/MED1 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human TRAP220
  Phe878-Phe1031
  Accession # Q15648
• Specificity: Detects endogenous human TRAP220 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAP220/MED1 Antibody [Alexa Fluor® 405]

• Activator-recruited cofactor 205 kDa component
• ARC205
• CRSP1TRIP2
• CRSP200p53 regulatory protein RB18A
• DRIP230
• DRIP230PPAR-binding protein
• MBD4
• MED1
• mediator complex subunit 1DRIP205
• mediator of RNA polymerase II transcription subunit 1
• PBPPPARGBP
• Peroxisome proliferator-activated receptor-binding protein
• PPAR binding protein
• PPARBP
• PPARBPMGC71488
• PPARG binding protein
• PPARGBP
• RB18ATR-interacting protein 2
• Thyroid hormone receptor-associated protein complex 220 kDa component
• thyroid hormone receptor-associated protein complex component TRAP220
• thyroid receptor interacting protein 2
• Thyroid receptor-interacting protein 2
• TRAP220
• TRAP220TRIP-2
• TRIP2
• vitamin D receptor-interacting protein 230 kD
• Vitamin D receptor-interacting protein complex component DRIP205

Background

TRAP220 (Thyroid hormone receptor associated protein 220 kDa) is a widely expressed member of the Mediator complex subunit 1 family of proteins. It constitutes one of some 25 subunits that make up the Mediator complex. Here, it serves as a scaffold protein that links nuclear hormone receptors to RNA polymerase II. Human TRAP220 is 1581 amino acids (aa) in length. It contains a Mediator complex interaction region (aa 108‑390) and two nuclear hormone receptor binding boxes (aa 600‑670). Phosphorylation of Thr1032 and Thr1457 materially increases TRAP220 activity. There are at least two splice variants. One shows a 22 aa substitution for aa 756‑1581 while another shows a nine aa substitution for aa 548‑1581. Over aa 878‑1031, human TRAP220 shares 92% aa identity with mouse TRAP220.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.