Recombinant Human TRAP1 Protein

SDS-Page: Recombinant Human TRAP1 Protein [NBP1-99222]

Bioactivity: Recombinant Human TRAP1 Protein [NBP1-99222] - SNX-0723 IC50 of 1.76 uM, SNX-0723 titration range (0.019-10 uM).

Recombinant Human TRAP1 Protein [NBP1-99222] - Geldanamycin IC50 of 1 nM.

Product Discontinued

Recombinant Human TRAP1 Protein Summary

• Description: Recombinant protein containing 702 amino acids for Human TRAP1
  
  

  Source:E. coli.
  Amino Acid Sequence:(Q12931)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TRAP1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: Kd = 1.95 nM. Assay conditions: 20 mM HEPES, pH 7.5, 50 mM KCl, 5 mM MgCl2, 20 mM sodium molybdate, 2 mM DTT, 0.01% NP40, 100 ug/ml BSA, 50 nM TRAP1, geldanamycin-FITC (titration range 0.016-16 nM) incubated at 4oC for 24 hrs. Fluorescence polarization measured at RT (Ex/Em= 485/520 nm)
• Theoretical MW: 76.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
• Concentration: 13.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human TRAP1 Protein

• HSP 75
• HSP75TNFR-associated protein 1
• HSP90Lheat shock protein 75 kDa, mitochondrial
• TNF receptor-associated protein 1
• TRAP-1
• tumor necrosis factor type 1 receptor associated protein
• Tumor necrosis factor type 1 receptor-associated protein

Background

Human TRAP1 belongs to the mitochondrial heat shock protein family. These molecular chaperones are highly conserved and play an important role in signal transduction, protein folding and degradation. Recombinant human TRAP1 has a C-terminal FLAG tag and has 702 amino acid residues. It has been identified to be protective for cell survival, and has been suggested as a target for anti-cancer therapeutics. Also can be useful for studies including enzyme kinetics, activator screening and selectivity profiling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TRAP1