TRAP alpha Antibody (OTI4C7)

Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP alpha.

Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP alpha.

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human thyroid tissue using anti-TRAP alpha mouse monoclonal antibody.

Flow Cytometry: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRAP alpha antibody, and then analyzed by flow cytometry.

Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP alpha monoclonal antibody.

Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody (4C7) [NBP2-01026] - Immunofluorescent staining of HeLa cells using anti-TRAP alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRAP alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRAP alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRAP alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TRAP alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRAP alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human endometrium tissue using anti-TRAP alpha mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human pancreas tissue using anti-TRAP alpha mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI4C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.95 mg/ml

TRAP alpha Antibody (OTI4C7) Summary

• Immunogen: Full length human recombinant protein of human SSR1(NP_003135) produced in HEK293 cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SSR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 32.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.95 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRAP alpha Antibody (OTI4C7)

• DKFZp781N23103
• FLJ14232
• FLJ22100
• FLJ23034
• FLJ78242
• FLJ93042
• signal sequence receptor, alpha
• SSR alpha subunit
• SSR-alpha
• translocon-associated protein alpha subunit
• translocon-associated protein subunit alpha
• TRAP alpha
• TRAP-alpha
• TRAPASignal sequence receptor subunit alpha

Background

The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAP alpha Antibody (NBP2-01026)(1)

Publication using NBP2-01026

• Losfeld ME, Ng BG, Kircher M et al. A new congenital disorder of glycosylation caused by a mutation in SSR4, the signal sequence receptor 4 protein of the TRAP complex. Hum Mol Genet. 2013-11-13 [PMID: 24218363] (WB, Human)

Review for TRAP alpha Antibody (NBP2-01026) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Marie-estelle LOSFELD WB Human 02/27/2014

Summary

• Application: Western Blot
• Sample Tested: human dermal fibroblasts
• Species: Human
• Lot: A01

Bioinformatics

• Gene Symbol: SSR1
• Entrez:
  • 6745(Human)