Transglutaminase 7/TGM7 Antibody

Western Blot: Transglutaminase 7/TGM7 Antibody [NBP1-68858] - (0.1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Transglutaminase 7/TGM7 Antibody Summary

• Description: Novus Biologicals Goat Transglutaminase 7/TGM7 Antibody (NBP1-68858) is a polyclonal antibody validated for use in WB and ELISA. Anti-Transglutaminase 7/TGM7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KIEISQGQGHSVT corresponding to internal region according to NP_443187.1.
• Epitope: C-KIEISQGQGHSVT
• Predicted Species: Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TGM7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in human lung and testis and in mouse kidney, lung and testis lysates (calculated MW of 79.9 kDa band according to human NP_443187.1 and 79.8 kDa band according to mouse NP_001153896.1). An additional band of unknown identity was also consistently observed at 17 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Transglutaminase 7/TGM7 Antibody

• protein-glutamine gamma-glutamyltransferase Z
• TG(Z)
• TGase Z
• TGase-7
• TGM7
• TGMZ
• TGMZEC 2.3.2.13
• TGZ
• Transglutaminase 7
• Transglutaminase Z
• transglutaminase-7

Background

Transglutaminases (TGM; EC 2.3.2.13) are a family of structurally and functionally related enzymes that stabilize protein assemblies through the formation of gamma-glutamyl-epsilon lysine crosslinks. Transglutaminases (TGM; EC 2.3.2.13) are a family of structurally and functionally related enzymes that stabilize protein assemblies through the formation of gamma-glutamyl-epsilon lysine crosslinks. For additional background information on transglutaminases, see TGM1 (MIM 190195).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-2110 AF363393.1 1-2110 2111-2312 BX112796.1 167-368.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transglutaminase 7/TGM7 Antibody (NBP1-68858)(1)

Publication using NBP1-68858

• Grenard P, Bates MK, Aeschlimann D. Evolution of transglutaminase genes: identification of a transglutaminase gene cluster on human chromosome 15q15. Structure of the gene encoding transglutaminase X and a novel gene family member, transglutaminase Z. J Biol Chem 2001-08-01 [PMID: 11390390]

Bioinformatics

• Gene Symbol: TGM7
• Entrez:
  • 116179(Human)
  • 640543(Mouse)
• Uniprot:
  • NP_443187.1()