Transglutaminase 3/TGM3 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

Transglutaminase 3/TGM3 Antibody [Alexa Fluor® 488] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 3/TGM3
  Ala2-Glu693
  Accession # NP_003236
• Specificity: Detects human Transglutaminase 3/TGM3 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Transglutaminase 3/TGM3 Antibody [Alexa Fluor® 488]

• EC 2.3.2.13
• MGC126250
• protein-glutamine gamma-glutamyltransferase E
• TG(E)
• TG3
• TGase E
• TGase-3
• TgaseE
• TGEMGC126249
• TGM3
• transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Transglutaminase 3
• Transglutaminase E
• transglutaminase-3

Background

Transglutaminase 3 (TG3), also known as epidermal Transglutaminase (Tgase E), belongs to the family of Transglutaminase enzymes that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). TG3 is involved in the formation of the cornified envelope in skin keratinocytes (4). It functions to cross-link structural proteins during epidermal terminal differentiation. TG3 has been implicated as the dominant autoantigen in dermatitis herpetiformis (5). TG3 activation requires proteolysis of the 77 kDa zymogen into two fragments of approximately 50 and 27 kDa to form the active enzyme (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.