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HAF016). For additional reference, Recombinant Human Transglutaminase 3/TGM3 (Catalog # 4604-TG) (10 ng/lane) uncleaved and cleaved by recombinant human Cathepsin were included. Specific bands were detected for uncleaved Transglutaminase 3/TGM3 at approximately 77 kDa (as indicated) and Transglutaminase 3/TGM3 cleavage products at approximately 50 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cells in the papillae layer in mucosa. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
Species: Hu Applications: WB, IHC Host: Sheep Polyclonal
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium dependent. Transglutaminase 3 consists of two polypeptide chains activated from a single precursor protein by proteolysis. Transglutaminase 3 is involved the later stages of cell envelope formation in the epidermis and hair follicle.
![Western Blot Transglutaminase 3/TGM3 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Transglutaminase_3_AF4604_Western_Blot_9826.jpg)
![Immunohistochemistry Transglutaminase 3/TGM3 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Transglutaminase_3_AF4604_Immunohistochemistry_10289.jpg)
