Transferrin Native Protein

Images

 
Dot Blot: Transferrin Native Protein [NBP1-96990] - Dot Blot results of Human Transferrin Biotin Conjugated. Antigen: Human Transferrin Biotin Conjugated. Blot loaded at 3-fold dilution: 1. 10 ng, 2. 33. ng, 3. 11. ng, ...read more
Dot Blot: Transferrin Native Protein [NBP1-96990] - Dot Blot of Biotin conjugated Human Transferrin. Antigen: Human Transferrin Biotin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications DB, ELISA, IB, ICC/IF, IHC
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates

Transferrin Native Protein Summary

Description
Native Transferrin protein.

Source: Human Serum

Preparation
Method
This product was prepared from normal serum by a multi-stage process that includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Transferrin and anti-Human Serum.
Protein/Peptide Type
Native Protein
Gene
TF
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Polyethylene Glycol (PEG-8000)
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunoelectrophoresis (IEP)
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Notes

For extended storage, aliquot reconstituted liquid and freeze at -20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Transferrin Native Protein

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

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Bioinformatics

Gene Symbol TF
Entrez