Transferrin Native Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC
Concentration
5.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Transferrin Native Protein Summary

Description
Native Rat Transferrin

Source: Normal serum (Accession #:P12346)
Preparation
Method
This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis against the buffer stated above. Rat Transferrin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Transferrin and anti-Rat Serum.
Protein/Peptide Type
Native Protein
Gene
TF
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Western Blot
Application Notes
This product is a suitable protein for use as a control reagent in both Western Blotting and ELISA experiments. For blotting, the expected molecular weight of Rat Transferrin is approximately 80kDa.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Preservative
No Preservative
Concentration
5.5 mg/ml
Purity
Multi-step

Notes

Store vial at 4C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product does not contain preservative. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Alternate Names for Transferrin Native Protein

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

Rat Transferrin is a blood plasma glycoprotein which binds iron and regulates the amount of available iron in circulation. Rat Transferrin has a high affinity for iron, but this binding is reversible. Rat Transferrin has a molecular weight of approximately 80 kDa. Rat Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Video Protocols

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Bioinformatics Tool for Transferrin Native Protein (NBP1-96775)

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Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

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Bioinformatics

Gene Symbol TF