Transferrin Overexpression Lysate

Images

 
There are currently no images for Transferrin Lysate (NBL1-16827).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Transferrin Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209184 and based on accession number NM_001063. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens transferrin (TF), mRNA.
Gene
TF

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
75.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Transferrin Overexpression Lysate

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

The protein encoded by this gene is a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds 1 ion of ferric iron. The function of this encoded protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NBP3-15868
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
MEP00B
Species: Mu
Applications: ELISA
NBP2-66881
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
236-EG
Species: Hu
Applications: BA
DCRP00
Species: Hu
Applications: ELISA
DTFP10
Species: Hu
Applications: ELISA
NB120-10109
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IHC-P, S-ELISA, WB
H00003077-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
D6050
Species: Hu
Applications: ELISA
NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-33320
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
202-IL
Species: Hu
Applications: BA
NBP1-58268
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBL1-16827
Species: Hu
Applications: WB

Publications for Transferrin Lysate (NBL1-16827) (0)

There are no publications for Transferrin Lysate (NBL1-16827).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Transferrin Lysate (NBL1-16827) (0)

There are no reviews for Transferrin Lysate (NBL1-16827). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Transferrin Lysate (NBL1-16827) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Transferrin Products

Bioinformatics Tool for Transferrin Lysate (NBL1-16827)

Discover related pathways, diseases and genes to Transferrin Lysate (NBL1-16827). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Transferrin Lysate (NBL1-16827)

Discover more about diseases related to Transferrin Lysate (NBL1-16827).
 

Pathways for Transferrin Lysate (NBL1-16827)

View related products by pathway.

PTMs for Transferrin Lysate (NBL1-16827)

Learn more about PTMs related to Transferrin Lysate (NBL1-16827).

Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Transferrin Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol TF