Human, Canine Transferrin ELISA Kit (Colorimetric)

ELISA: Transferrin ELISA Kit (Colorimetric) [NBP2-61298] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Hu, Ca
• Applications: ELISA
• Suitable Sample Type: human serum, human plasma and canine serum
• Standard Curve Range: 4.9 - 5000 ng/ml
• Sensitivity: 4.6 ng/ml

Human, Canine Transferrin ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 4.9 - 5000 ng/ml
• Sensitivity: 4.6 ng/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Mean 14.1%
• Intra-Assay: Mean 6.4%
• Spike Recovery: Human Serum - 83%
  Human EDTA Plasma - 110%
  Canine Serum - 143%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TF

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human, Canine Transferrin ELISA Kit (Colorimetric)

• Beta-1 metal-binding globulin
• DKFZp781D0156
• EC 3.4.21
• PRO1557
• PRO2086
• Serotransferrin
• Siderophilin
• TF
• Transferrin

Background

Human serum transferrin, one of the members of the family of transferrin proteins, is an 80kDa glycoprotein and is a major carrier of iron in blood. Also included in this family of proteins are: i) lactoferrin, which is the iron-binding protein found in human milk and ii) ovotransferrin, which is the iron-binding protein isolated from avian egg white. Transferrin binds iron, forming a transferrin-iron complex. When this complex encounters a transferrin receptor on the surface of the cell, it binds and is transported into the cytoplasm. Once inside the cell, the iron is released and the iron- free transferrin, now referred to as apotransferrin, is transported back to the cell surface. Each molecule of transferrin can bind up to two atoms of iron to form reddish protein-iron complexes. Transferrin allows for the transport of the ferric iron (Fe3+) from the intestine, reticuloendothelial system and liver parenchymal cells to all cells in the body. Measurements of serum transferrin has applications in anemia, where medical professionals may check transferrin levels in iron deficiency, as an increase in transferrin levels may be seen in patients with iron deficiency anemia. Additionally, transferrin has a role in iron overload disorders such as hemochromatosis, where transferrin levels would be decreased in plasma and serum. Decreased levels of transferrin can also be detected in patients with liver diseases, such as cirrhosis and non-alcoholic fatty liver disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Transferrin ELISA Kit (NBP2-61298). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TF