Rat Transferrin ELISA Kit (Colorimetric)

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ELISA: Rat Transferrin ELISA Kit (Colorimetric) [KA1847] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Plasma, Serum
Standard Curve Range
0.156 to 5 ug/mL
Sensitivity
0.1 ug/mL

Order Details

Rat Transferrin ELISA Kit (Colorimetric) Summary

Description
Tf (Rat) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of rat Tf.
Standard Curve Range
0.156 to 5 ug/mL
Sensitivity
0.1 ug/mL
Assay Type
Competitive ELISA
Inter-Assay
CV% < 7%
Intra-Assay
CV% < 4.5%
Spike Recovery
95%
Sample Volume
25 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TF

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publications using KA1847.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Transferrin: lyophilized.
  2. Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
  3. MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  4. Rat Transferrin Microplate: A 96-well polystyrene microplate (12 strips of 8 wells)coated with a polyclonal antibody against rat Transferrin.
  5. Rat Transferrin Standard: Rat Transferrin in a buffered protein base, lyophilized.
  6. Sealing Tapes: pressure-sensitive sealing tapes that can be cut to fit the format of theindividual assay.
  7. Stop Solution: A 0.5 N hydroxychloric acid to stop the chromogen substrate reaction.
  8. Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  9. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rat Transferrin ELISA Kit (Colorimetric)

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Transferrin ELISA Kit (KA1847)(2)

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Research Areas for Transferrin ELISA Kit (KA1847)

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Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

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Bioinformatics

Gene Symbol TF