Human TRAILR3/TNFRSF10C ELISA Kit (Colorimetric) Summary
| Description |
Incubation: 3 hours 40 minutes |
| Specificity |
Specific for human TRAILR3/TNFRSF10C. No cross reactivity was observed with TRAIL, CD117, IL-6R, IL-2R, CD116, TRAIL R1, TRAIL R2, TRAIL R4, CD178, and Granzyme B.. |
| Standard Curve Range |
312.5-10000 pg/ml |
| Sensitivity |
147 pg/ml |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 9.7 |
| Intra-Assay |
CV% < 6.8 |
| Spike Recovery |
103 to 122% |
| Sample Volume |
100 ul |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
TNFRSF10C |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- Biotinylated secondary antibody
- Buffers
- Controls (where applicable)
- Detailed procedure.
- Pre-coated strip plates
- Standards
- Stop reagents
- Streptavidin-HRP
- TMB
|
Alternate Names for Human TRAILR3/TNFRSF10C ELISA Kit (Colorimetric)
Background
DcR1 / TRAIL-R3 / TRID / LIT is one of the two putative decoy receptors identified for TRAIL, one of the members of TNF family of apoptosis inducing proteins. The other putative decoy receptor is called DcR2 / TRUNDD / TRAIL-R4. DcR1 is attached to the cell surface through glycophospholipid anchor. It has the extracellular TRAIL binding domain but lacks the cytoplasmic domain to induce apoptotic signal. Hence overexpression of DcR1 inhibits the TRAIL induced apoptosis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for TRAILR3/TNFRSF10C ELISA Kit (NBP3-14638). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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