TRAIL/TNFSF10 Antibody

Western Blot: TRAIL/TNFSF10 Antibody [NBP2-23644] - Human brain tissue lysate. (A) 2.5 and (B) 5 ug/ml.

Immunohistochemistry-Paraffin: TRAIL/TNFSF10 Antibody [NBP2-23644] - Human brain tissue with TRAIL antibody at 20 ug/mL.

Western Blot: TRAIL/TNFSF10 Antibody [NBP2-23644] - Analysis of TRAIL in HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with TRAIL antibody at 1 ug/mL.

Immunofluorescence: TRAIL/TNFSF10 Antibody [NBP2-23644] - Immunofluorescence of TRAIL in human brain tissue with Trail antibody at 20 ug/mL.Red: Trail Antibody (NBP2-23644). Blue: DAPI staining.

Product Discontinued

TRAIL/TNFSF10 Antibody Summary

• Immunogen: Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TRAIL. The immunogen is located within the last 50 amino acids of Trail. Amino Acid Squence: TNEHLIDMDHEASFFGA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNFSF10
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 20 ug/ml
  • Immunohistochemistry-Paraffin 20 ug/ml
  • Western Blot 1-5 ug/ml
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Purity: Peptide affinity purified

Alternate Names for TRAIL/TNFSF10 Antibody

• Apo-2 ligand
• APO2L
• Apo-2Ltumor necrosis factor (ligand) family, member 10
• APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
• CD253 antigen
• CD253
• Protein TRAIL
• TL2
• TNF-related apoptosis-inducing ligand
• TNFSF10
• TRAIL
• TRAILTNF-related apoptosis inducing ligand TRAIL
• tumor necrosis factor (ligand) superfamily, member 10
• tumor necrosis factor ligand superfamily member 10

Background

Apoptosis or programmed cell death is induced in cells by a group of death domain containing receptors. Binding of ligand to these receptors sends signals that activate members of the caspase family of proteases. The signals ultimately cause degradation of chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis induced ligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors, DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and a cytoplasmic "death domain". In addition, two decoy receptors for TRAIL have also been identified. These receptors, designated DcR1 and DcR2, lack the death domain. Binding of TRAIL to either of these receptors, therefore, does not transmit the death signal. Thus, these receptors represent a novel way of regulating cell sensitivity to a pro-apoptotic cytokine at the cell surface. TRAIL is expressed predominantly in spleen, lung, and prostate but also in many other tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF10