TRAF3IP2 Antibody

Western Blot: TRAF3IP2 Antibody [NBP2-26102] - Staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRAF3IP2 Antibody Summary

• Immunogen: Peptide with sequence C-PDTGHDSDKSDQ corresponding to internal region according to NP_679211.1, NP_001157753.1.
• Specificity: This antibody is expected to recognise reported isoforms 2 and 3 (NP_679211.1; NP_001157753.1).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRAF3IP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 65 kDa band observed in lysates of MCF7 (calculated MW of 63.6 kDa band according to NP_679211.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRAF3IP2 Antibody

• ACT1DKFZP586G0522
• adapter protein CIKS
• C6orf2
• C6orf4chromosome 6 open reading frame 5
• C6orf5DKFZp586G0522
• C6orf6MGC3581
• CIKSchromosome 6 open reading frame 2
• Connection to IKK and SAPK/JNK
• NFkB-activating protein ACT1
• Nuclear factor NF-kappa-B activator 1
• TRAF3 interacting protein 2
• TRAF3-interacting protein 2

Background

TRAF3IP2 encodes a protein involved in regulating responses to cytokines by members of the Rel/NF-kappaB transcription factor family. These factors play a central role in innate immunity in response to pathogens, inflammatory signals and stress. This gene product interacts with TRAF proteins (tumor necrosis factor receptor-associated factors) and either I-kappaB kinase or MAP kinase to activate either NF-kappaB or Jun kinase. Two alternative transcripts encoding different proteins have been identified. A third transcript, which does not encode a protein and is transcribed in the opposite orientation, has been identified. Overexpression of this transcript has been shown to reduce expression of at least one of the protein encoding transcripts, suggesting it has a regulatory role in the expression of this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF3IP2 Antibody (NBP2-26102)(1)

Publication using NBP2-26102

• Qian Y, Liu C, Hartupee J et al. The adaptor Act1 is required for interleukin 17-dependent signaling associated with autoimmune and inflammatory disease. Nat Immunol 2007-03-01 [PMID: 17277779]

Bioinformatics

• Gene Symbol: TRAF3IP2
• Entrez:
  • 10758(Rat)
  • 10758(Rat)