TRAF-6 Antibody (326019) [Unconjugated]

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human TRAF-6 Monoclonal Antibody (Catalog # MAB3284, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 326019
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TRAF-6 Antibody (326019) [Unconjugated] Summary

• Immunogen: E.coli-derived recombinant human TRAF-6
  Met1-Val522
  Accession # Q9Y4K3
• Specificity: Detects human TRAF-6 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAF-6 Antibody (326019) [Unconjugated]

• E3 ubiquitin-protein ligase TRAF6
• EC 6.3.2.-
• Interleukin-1 signal transducer
• RING finger protein 85
• RNF85MGC:3310
• TNF receptor-associated factor 6
• TRAF6
• TRAF-6

Background

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs2-6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-6 is a 522 amino acid, 60 kDa protein. Overexpression of TRAF-6 mediates the activation of NF-kappa-B and JNK signaling pathways. TRAF-3 and TRAF-6 have been identified as adaptor molecules involved in TLR/IL-1R signaling events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-6 Antibody (MAB3284)(1)

Publication using MAB3284

• Jamie Frankish, Debayan Mukherjee, Erminia Romano, Katharina Billian-Frey, Matthias Schröder, Karl Heinonen et al. The CD40 agonist HERA-CD40L results in enhanced activation of antigen presenting cells, promoting an anti-tumor effect alone and in combination with radiotherapy Frontiers in Immunology 2023-05-26 [PMID: 37304285]

Bioinformatics

• Uniprot:
  • Q9Y4K3()