TRAF-5 Antibody

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TRAF‑5 was detected in immersion fixed paraffin-embedded sections of human lymph node using Sheep Anti-Human TRAF‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3280) at 3 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

TRAF-5 Antibody Summary

Immunogen
E. coli-derived recombinant human TRAF-5
Lys353-Leu557
Accession # O00463
Specificity
Detects human TRAF-5 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) TRAF-3 is observed, and less than 5% cross-reactivity with rhTRAF-2 and rhTRAF-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TRAF5
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
Control
TRAF-5 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAF-5 Antibody

  • MGC:39780
  • RNF84
  • RNF84RING finger protein 84
  • TNF receptor-associated factor 5
  • TRAF5
  • TRAF-5

Background

TRAF-5 (TNF Receptor-Associated Factor 5; also RNF84) is a 60-66 kDa member of the A subfamily of the TRAF family of molecules. It is widely expressed, and serves as an adaptor molecule that links select TNFR family members with downstream signaling elements. In particular, it interacts with LTbR, TNFR2, OX40, CD27, CD40, CD30, RANK and TAJ/TROY, and induces NF kappa B activation. It forms homotrimers and heteromers with TRAF3. Human TRAF5 is 557 amino acids (aa) in length. It contains one RING-type Zn finger region (aa 45-85), two TRAF-type Zn finger regions (aa 127-181 and 182-239), a coiled-coil region (aa 237-342) and a C-terminal protein-protein interaction MATH domain (aa 403-549). There is one potential alternative start site 64 aa upstream of the standard start site. Full-length human TRAF-5 shares 80% aa sequence identity with mouse TRAF-5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRAF5
Uniprot