TRACP/PAP/ACP5 Antibody

Western Blot: TRACP/PAP/ACP5 Antibody [NBP1-31535] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAP antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: TRACP/PAP/ACP5 Antibody [NBP1-31535] - Mouse intestine tissue. TRAP antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: TRACP/PAP/ACP5 Antibody [NBP1-31535] - NIH-3T3, with 10% SDS PAGE, diluted at 1:1000.

Western Blot: TRACP/PAP/ACP5 Antibody [NBP1-31535] - Sample(30 ug whole cell lysate) A:A431 B:MOLT4 12% SDS PAGE, antibody diluted at 1:1000.

Product Discontinued

TRACP/PAP/ACP5 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TRAP. The exact sequence is proprietary.
• Localization: Lysosome
• Predicted Species: Rhesus Macaque (97%), Canine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACP5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rabbit (86%), Sheep (85%), Rat (87%), Pig (86%), Bovine (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for TRACP/PAP/ACP5 Antibody

• ACP5
• HPAP
• TRACP
• TRACP5a
• TRACP5b
• TRACP-5b
• TRAP
• TrATPase
• TTRRAP

Background

The gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ACP5