TRACP/PAP/ACP5 Antibody (417603) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clone: 417603
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

TRACP/PAP/ACP5 Antibody (417603) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TRACP/PAP/ACP5
  Ala22-Pro320
  Accession # P13686
• Specificity: Detects human TRACP in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRACP/PAP/ACP5 Antibody (417603) [Alexa Fluor® 350]

• ACP5
• HPAP
• TRACP
• TRACP5a
• TRACP5b
• TRACP-5b
• TRAP
• TrATPase
• TTRRAP

Background

Encoded by the ACP5 gene, Tartrate Resistant Acid Phosphatase (TRACP or TRAP) is also known as Purple Acid Phosphatase (PAP) or Acid Phosphatase 5 (ACP5) (1). The deuced amino acid (aa) sequence of human ACP5 predicts a signal peptide (aa 1-21) and a mature chain (aa 22-325). R&D Systems’ recombinant human ACP5 consists of aa 22-320, without the last 5 residues, RRARP.

ACP5 is expressed at high levels by osteoclasts, macrophages and dendritic cells (2). Two forms, 5a and 5b, circulating in human blood, are derived from different cell types and have different functions. Derived from macrophages and dendritic cells, 5a is a marker of inflammatory conditions. Derived from osteoclasts, 5b is a marker of bone resorption. Compared to 5a, 5b does not contain sialic acid residues, has a higher specific activity and pH optimum, and may be processed into a disulfide-linked dimer (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.