TRA2A Antibody

Knockdown Validated: TRA2A Antibody [H00029896-B01P] - Western blot analysis showing depletion of TRA2B induces reciprocal upregulation of Tra2a protein expression, whereas depletion of TRA2A had minimal effect on Tra2b protein expression. Image collected and cropped by CiteAb from the following publication (nature.com/articles/ncomms5760), licensed under a CC-BY licence.

Western Blot: TRA2A Antibody [H00029896-B01P] - Analysis of TRA2A expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: TRA2A Antibody [H00029896-B01P] - Analysis of purified antibody to TRA2A on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: TRA2A Antibody [H00029896-B01P] - Analysis of TRA2A expression in HepG2 .

Western Blot: TRA2A Antibody [H00029896-B01P] - Analysis of TRA2A expression in Raw 264.7.

Western Blot: TRA2A Antibody [H00029896-B01P] - Analysis of TRA2A expression in transfected 293T cell line by TRA2A polyclonal antibody. Lane 1: TRA2A transfected lysate(31.13 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, KD
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated

TRA2A Antibody Summary

• Immunogen: TRA2A (AAH17094, 1 a.a. - 282 a.a.) full-length human protein. MSDVEENNFEGRESRSQSKSPTGTPARVKSESRSGSRSPSRVSKHSESHSRSRSKSRSRSRRHSHRRYTRSRSHSHSHRRRSRSRSYTPEYRRRRSRSHSPMSNRRRHTGSRANPDPNTCLGVFGLSLYTTERDLREVFSRYGPLSGVNVVYDQRTGRSRGFAFVYFERIDDSKEAMERANGMELDGRRIRVDYSITKRAHTPTPGIYMGRPTHSGGGGGGGGGGGGGGGGRRRDSYYDRGYDRGYDRYEDYDYRYRRRSPSPYYSRYRSRSRSRSYSPRRY
• Specificity: TRA2A - transformer-2 alpha,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: TRA2A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It is also useful for immunofluoresence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRA2A Antibody

• HSU53209
• htra-2 alpha
• htra-2-alpha
• putative MAPK activating protein PM24
• TRA-2 alpha
• tra2a
• Tra2alpha
• TRA2-alpha
• transformer 2 alpha homolog (Drosophila)
• transformer-2 alpha
• Transformer-2 protein homolog A
• transformer-2 protein homolog alpha

Background

This gene is a member of the transformer 2 homolog family and encodes a protein with two RS domains and an RRM (RNA recognition motif) domain. This phosphorylated nuclear protein binds to specific RNA sequences and plays a role in the regulation of pre-mRNA splicing. Several alternatively spliced transcript variants of this gene have been described; however, the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA2A Antibody (H00029896-B01P)(1)

Publication using H00029896-B01P

• Best A, James K, Dalgliesh C et al. Human Tra2 proteins jointly control a CHEK1 splicing switch among alternative and constitutive target exons. Nat Commun. 2014-09-11 [PMID: 25208576]

Bioinformatics

• Gene Symbol: TRA2A
• Entrez:
  • 29896(Human)
• Uniprot:
  • AAH17094(Human)