TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free

Western Blot: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986] - Analysis of NB100-1833 expression in NTERA-2 cl.D1 whole cell lysate. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # NB100-1833) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgM Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Image from the standard format of this antibody.

Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-1833. Staining of human cancer line PSN1. Image from verified customer review.

• Reactivity: Hu, Mu(-)
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: TRA-1-81
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free (NB100-1833) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human embryonal carcinoma cell line 2102Ep.
• Localization: Cell Membrane
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000. Use reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500

Reactivity Notes

This antibody recognizes the TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. This antibody recognizes antigens that are associated with a pericellular matrix proteoglycan.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: IgM purified

Alternate Names for TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free

• GCTM-2 antigen
• GP135
• gp200
• PC
• PCLP
• PCLP-1
• PDX
• Podocalyxin
• Podocalyxin-like protein 1
• PODXL1
• TRA181
• TRA-1-81

Background

TRA-1-81 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.