TRA-1-81 Antibody (TRA-1-81)


Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis of NB100-1833 expression in NTERA-2 cl.D1 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog more
Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-1833. Staining of human cancer line PSN1. Image from verified customer review.

Product Details

Reactivity Hu, Mu(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IP
1 mg/ml

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TRA-1-81 Antibody (TRA-1-81) Summary

Human embryonal carcinoma cell line 2102Ep.
Cell Membrane
Embryonic Stem Cell Marker, Tumor Vessel Marker
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1:10-1:1000. Use reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
Read Publications using
NB100-1833 in the following applications:

Reactivity Notes

This antibody recognizes the TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. This antibody recognizes antigens that are associated with a pericellular matrix proteoglycan.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1 mg/ml
IgM purified

Alternate Names for TRA-1-81 Antibody (TRA-1-81)

  • gp200
  • MGC138240
  • PC
  • PCLP
  • TRA181
  • TRA-1-81


TRA-1-81 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA-1-81 Antibody (NB100-1833)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, ICC/IF, WB.

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Showing Publications 1 - 7 of 7.
Publications using NB100-1833 Applications Species
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector. Stem Cell Research. 2016-03-01 [PMID: 28677531] (ICC/IF, Human)

This citation used the DyLight 650 form of this antibody.
ICC/IF Human
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector Stem Cell Research 2016-01-01 [PMID: 27638042] (ICC/IF, Human)

This reference used the DyLight 650 version of NB100-1833.
ICC/IF Human
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector. Stem Cell Res 2016-06-28 [PMID: 27345993] (ICC/IF, Human) ICC/IF Human
Hildebrand L, Seemann P, Kurtz A et al. Selective cell targeting and lineage tracing of human induced pluripotent stem cells using recombinant avian retroviruses Cell. Mol Life Sci 2015-06-25 [PMID: 26109426] (ICC/IF, Human)

This publication used the DL650 conjugated form of this antibody (NB100-1833C)
ICC/IF Human
Mizrak SC, Chikhovskaya JV, Sadri-Ardekani H et al. Embryonic stem cell-like cells derived from adult human testis. Hum Reprod :dep354. 2009-01-01 [PMID: 19815622] (ICC/IF, Human) ICC/IF Human
Henderson, JK et al. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells;20(4):329-37. 2002-01-01 [PMID: 12110702] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Badcock, G et al. The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res;59(18):4715-9. 1999-09-15 [PMID: 10493530] (WB, Human) WB Human

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Product General Protocols

View specific protocols for TRA-1-81 Antibody (NB100-1833): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRA-1-81 Antibody (NB100-1833). (Showing 1 - 1 of 1 FAQs).

  1. We would like to know the host of the following antibodies, is BALC/c mouse or not?
    • The lab has informed me that these were both produced in BALB/c mice.

Secondary Antibodies


Isotype Controls

Additional TRA-1-81 Products

Array NB100-1833

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