TRA-1-81 Antibody (TRA-1-81)

Images

 
Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis of NB100-1833 expression in NTERA-2 cl.D1 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog ...read more
Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-1833. Staining of human cancer line PSN1. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
TRA-1-81
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price

TRA-1-81 Antibody (TRA-1-81) Summary

Immunogen
Human embryonal carcinoma cell line 2102Ep.
Localization
Cell Membrane
Marker
Embryonic Stem Cell Marker, Tumor Vessel Marker
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
Application Notes
This TRA-1-81 antibody is useful for Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot. Flow Cytometry was reported in scientific literature.
Publications
Read Publications using
NB100-1833 in the following applications:

Reactivity Notes

Human. This antibody recognizes the TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. This antibody recognizes antigens that are associated with a pericellular matrix proteoglycan.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
IgM purified

Alternate Names for TRA-1-81 Antibody (TRA-1-81)

  • gp200
  • MGC138240
  • PC
  • PCLP
  • PODXL
  • TRA181
  • TRA-1-81

Background

TRA-1-81 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA-1-81 Antibody (NB100-1833)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, ICC/IF, WB.


Filter By Application
FLOW
(1)
ICC/IF
(6)
WB
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-1833 Applications Species
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector. Stem Cell Research. 2016 Mar 01 [PMID: 28677531] (ICC/IF, Human)

Details:
This citation used the DyLight 650 form of this antibody.
ICC/IF Human
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector Stem Cell Research 2016 Mar 01 (ICC/IF, Human) [PMID: 27638042] (ICC/IF, Human)

Details:
This reference used the DyLight 650 version of NB100-1833.
ICC/IF Human
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector Stem Cell Research [PMID: 27345993] (ICC/IF, Human)

Details:
This publication used the DyLight 650 conjugated form of this antibody (Cat# NB100-1833C).
ICC/IF Human
Hildebrand L, Seemann P, Kurtz A et al. Selective cell targeting and lineage tracing of human induced pluripotent stem cells using recombinant avian retroviruses Cell. Mol Life Sci 2015 Jun 25 [PMID: 26109426] (ICC/IF, Human)

Details:
This publication used the DL650 conjugated form of this antibody (NB100-1833C)
ICC/IF Human
Mizrak SC, Chikhovskaya JV, Sadri-Ardekani H et al. Embryonic stem cell-like cells derived from adult human testis. Hum Reprod :dep354. 2009 [PMID: 19815622] (ICC/IF, Human) ICC/IF Human
Henderson, JK et al. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells;20(4):329-37. 2002 [PMID: 12110702] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
Badcock, G et al. The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res;59(18):4715-9. 1999 Sep 15. [PMID: 10493530] (WB, Human) WB Human

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Product General Protocols

View specific protocols for TRA-1-81 Antibody (NB100-1833): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRA-1-81 Antibody (NB100-1833). (Showing 1 - 1 of 1 FAQs).

  1. We would like to know the host of the following antibodies, is BALC/c mouse or not?
    • The lab has informed me that these were both produced in BALB/c mice.

Secondary Antibodies

 

Isotype Controls

Additional TRA-1-81 Products

Array NB100-1833

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