TR4/NR2C2 Antibody (JE45-03)

Western Blot: TR4/NR2C2 Antibody (JE45-03) [NBP3-33040] - Western blot analysis of TR4/NR2C2 on different lysates with Rabbit anti-TR4/NR2C2 antibody (NBP3-33040) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Jurkat cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33040) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TR4/NR2C2 Antibody (JE45-03) [NBP3-33040] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TR4/NR2C2 antibody (NBP3-33040) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33040) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: JE45-03
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TR4/NR2C2 Antibody (JE45-03) Summary

• Description: Novus Biologicals Rabbit TR4/NR2C2 Antibody (JE45-03) (NBP3-33040) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human TR4/NR2C2 aa 497-596 / 596. (Uniprot: P49116)
• Localization: Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NR2C2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:1000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TR4/NR2C2 Antibody (JE45-03)

• hTAK1
• NR2C2
• Nuclear hormone receptor TR4
• nuclear receptor subfamily 2 group C member 2
• nuclear receptor subfamily 2, group C, member 2
• Orphan nuclear receptor TAK1
• Orphan nuclear receptor TR4
• TAK1
• TAK1TR4TR2R1
• Testicular receptor 4
• TR4 nuclear hormone receptor
• TR4

Background

TR4 (TAK1, NR2C2) is an orphan nuclear receptor. TR4 was originally cloned from lymphoblastoma Raji cells or mouse brain cDNA library. No ligand has been reported. Northern blot shows TR4 is transcribed as a 9kb mRNA in many tissues and as a 2.8kb mRNA in testis, mainly in spermatocytes. TR4 has two isoforms called TR4alpha1 and TR4 alpha2,which differ in 19 amino acids coded by two separate exons. Both products translated from the 9kb transcript are ubiquitously expressed. Since TR4 binds to the same elements for the RAR-RXR or TR-RXR heterodimers, TR4 may have an inhibitory effect for retinoic-acid mediated transactivation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR2C2