TPX2 Antibody (18D5)

Images

 
Western Blot: TPX2 Antibody (18D5) [NB100-78473] - MOLT-4 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TPX2 (clone 18D5) antibody. Proteins were ...read more
Immunocytochemistry/ Immunofluorescence: TPX2 Antibody (18D5) [NB100-78473] - Staining of HeLa cells. Photo courtesy of product review by verified customer.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78473
    • Availability
      Product Discontinued

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TPX2 Antibody (18D5) Summary

Immunogen
Amino Acid: 1-220 of human TPX2
Specificity
TPX2 (18D5)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TPX2
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel
Positive Control
TPX2 Lysate (NBL1-17236)
Reviewed Applications
Read 1 Review rated 5
using
NB100-78473 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for TPX2 Antibody (18D5)

  • C20orf1HCTP4
  • C20orf2
  • chromosome 20 open reading frame 1
  • Differentially expressed in cancerous and non-cancerous lung cells 2
  • differentially expressed in lung cells
  • DIL2
  • DIL-2FLS353
  • HCA519
  • Hepatocellular carcinoma-associated antigen 519
  • P100
  • p100GD:C20orf1
  • preferentially expressed in colorectal cancer
  • Protein fls353
  • REPP86
  • restricted expression proliferation associated protein 100
  • Restricted expression proliferation-associated protein 100
  • targeting protein for Xklp2
  • TPX2, microtubule-associated protein homolog
  • TPX2, microtubule-associated, homolog (Xenopus laevis)

Background

The TPX2 protein, also known as targeting protein for Xklp1, restricted expression proliferation associated protein p100, and differentially expressed in cancer and non-cancerous lung cells protein 2 (DIL2), is a 100 kD nuclear protein that contains two coiled-coil domains. The TPX2 protein is strictly associated with spindle pole and mitotic spindle during mitosis. In the G2/S position of the cell cycle, TPX2 is diffusely distributed throughout nucleus. TPX2 has been shown to be highly expressed in lung carcinomas cell lines, but not in normal lung tissues. TPX2 is thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays and may also be involved in targeting Aurora-A kinase to the mitotic spindle. TPX2 has been shown to interact with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, alpha-tubulin, and nucleolin among others. TPX2 can be modified by phosphorylation on serine 738. The 18D5 monoclonal antibody recognizes human TPX2 and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TPX2 Antibody (NB100-78473) (0)

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Review for TPX2 Antibody (NB100-78473) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB100-78473:
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IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence TPX2 NB100-78473
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reviewed by:
Helen Chen
IF Human 01/09/2013
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Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells
SpeciesHuman
CommentsThe TPX2 antibody works great, though I did notice some cross-reactivity when it's costained with the TUBG1 antibody from Sigma (T6557), I guess to be more accurate, the TUBG1 is showing cross-reactivity with the TPX2 antibody.

Blocking

Blocking Details3% BSA in PBS-Tween 20 at room temperature for 1hr

Primary Anitbody

Dilution Ratio1:10 dilution at room temperature for 1hr in 1.5% BSA in PBS-Tween 20

Secondary Antibody

Secondary DescriptionGoat anti-mouse
Secondary Manufacturer Cat#Alexa Fluor 488
Secondary Concentration1:2000

Details

Detection NotesOlympus Fluoview 10i Confocal
Fixation DetailsMethanol fixation at -20C for 15mins
Wash Description3 washes in PBS for 10mins each

Comments

CommentsThe TPX2 antibody works great, though I did notice some cross-reactivity when it's costained with the TUBG1 antibody from Sigma (T6557), I guess to be more accurate, the TUBG1 is showing cross-reactivity with the TPX2 antibody.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TPX2 Antibody (NB100-78473) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
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2
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1
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Helen Chen
01/09/2013
Application: IF
Species: Human

Bioinformatics

Gene Symbol TPX2
Entrez