TPR Antibody (1A8) - Azide and BSA Free

Western Blot: TPR Antibody (1A8) [H00007175-M01] - TPR expression in Hela NE ( Cat # L013V3 ).

Immunohistochemistry-Paraffin: TPR Antibody (1A8) [H00007175-M01] - Analysis of monoclonal antibody to TPR on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml.

Sandwich ELISA: TPR Antibody (1A8) [H00007175-M01] - Detection limit for recombinant GST tagged TPR is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Pm
• Applications: WB, ELISA, IHC
• Clone: 1A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TPR Antibody (1A8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TPR (NP_003283, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAVLQQVLERTELNKLPKSVQNKLEKFLADQQSEIDGLKGRHEKFKVESEQQYFEIEKRLSHSQERLVNETRECQSLRLELEKLNNQLKALTEKNKE
• Specificity: TPR - translocated promoter region (to activated MET oncogene)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TPR Antibody (1A8) - Azide and BSA Free

• nuclear pore complex-associated protein TPR
• nucleoprotein TPR
• translocated promoter region (to activated MET oncogene)
• tumor potentiating region

Background

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TPR Antibody (H00007175-M01)(4)

Publications using H00007175-M01

• Duheron V, Chatel G, Sauder U et al. Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy. Nucleus 2014-01-01 [PMID: 25485891] (WB)
• Copeland AM, Newcomb WW, Brown JC. Herpes Simplex Virus Replication: Roles of Viral Proteins and Nucleoporins in Capsid-Nucleus Attachment. J Virol. 2008-12-10 [PMID: 19073727]
• Cherian-Shaw M, Puttabyatappa M, Greason E et al. Expression of SR-BI LDL receptor differential use of lipoproteins to support early steroidogenesis in luteinizing macaque granulosa cells. Endocrinology :en2008-0619. 2008-01-01 [PMID: 18832102]
• 1 Chan EY, Qian WJ, Diamond DL et al. Quantitative Analysis of HIV-1 Infected CD4+ Cell Proteome: Dysregulated Cell Cycle Progression Nuclear Transport Coincide with Robust Virus Production. J Virol;81(14):7571-83. 2007-07-01 [PMID: 17494070]

Bioinformatics

• Gene Symbol: TPR
• Entrez:
  • 7175(Human)
• OMIM:
  • 189940(Human)
• Uniprot:
  • NP_003283(Human)