TPPP/p25 Antibody

Images

 
Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - (2ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) Analysis of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

TPPP/p25 Antibody Summary

Immunogen
Peptide with sequence C-DPSKDRAAKRLSLES corresponding to internal region according to NP_008961.1.
Epitope
DPSKDRAAKRLSLES - Internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TPPP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 26 kDa band observed in human brain is reported in scientific literature (PMID: 18028908). An additional band of unknown identity was also consistently observed at 17 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human cerebral cortex shows cytoskeletal-like staining throughout the fibre network.
Publications
Read Publications using NBP1-49833.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TPPP/p25 Antibody

  • p24
  • P24,25 kDa brain-specific protein
  • p25
  • p25alpha
  • p25-alpha
  • TPPP/p25brain specific protein p25 alpha
  • TPPP1glycogen synthase kinase 3 (GSK3) inhibitor p24
  • tubulin polymerization promoting protein
  • tubulin polymerization-promoting protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TPPP/p25 Antibody (NBP1-49833)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TPPP
Entrez
Uniprot