TORC2 Antibody (628430)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, MDA-MB-453 human breast cancer cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse TORC2 Monoclonal Antibody (Catalog # MAB6338) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TORC2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 628430
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TORC2 Antibody (628430) Summary

• Immunogen: E. coli-derived recombinant human TORC2
  Lys453-Ser613
  Accession # Q53ET0
• Specificity: Detects human and mouse TORC2 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRTC2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.01 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TORC2 Antibody (628430)

• CREB regulated transcription coactivator 2
• CREB-regulated transcription coactivator 2
• CRTC2
• TORC2
• TORC-2
• TORC2transducer of regulated cAMP response element-binding protein (CREB) 2
• Transducer of CREB protein 2
• Transducer of regulated cAMP response element-binding protein 2

Background

TORC2, also known as CREB-regulated transcription co-activator 1 (crtc2), is an approximately 75 kDa protein that functions as a co‑activator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC2 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its O-glycosylation triggers TORC2-dependent induction of hepatic gluconeogenic enzymes. It mediates hypothalamic glucose sensing and also the sensing of endoplasmic reticulum stress. In addition, TORC2 contributes to the transcription of HTLV-1 genes and the activation of latent EBV infections. Within aa 453‑613, human TORC2 shares 84% aa sequence identity with mouse TORC2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TORC2 Antibody (MAB6338)(1)

Publication using MAB6338

• T Yoshimoto, M Kittaka, AAP Doan, R Urata, M Prideaux, RE Rojas, CV Harding, W Henry Boom, LF Bonewald, EM Greenfield, Y Ueki Osteocytes directly regulate osteolysis via MYD88 signaling in bacterial bone infection Nature Communications, 2022-11-04;13(1):6648. 2022-11-04 [PMID: 36333322] (Mouse)

Bioinformatics

• Gene Symbol: CRTC2
• Uniprot:
  • Q53ET0(Human)