TORC1 Antibody

Western Blot: TORC1 Antibody [NB100-2352] - Detection of human TORC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TORC1 antibody NB100-2352 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: TORC1 Antibody [NB100-2352] - Detection of human TORC1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-TORC1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: TORC1 Antibody [NB100-2352] - Detection of Human and Mouse TORC1 on HeLa whole cell lysate using NB100-2352. TORC1 was also immunoprecipitated by another rabbit anti-TORC1 antibody and NB100-2351.

Immunohistochemistry: TORC1 Antibody [NB100-2352] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TORC1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

TORC1 Antibody [NB100-2352] - Detection of mouse TORC1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TORC1 antibody NB100-2352 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

TORC1 Antibody [NB100-2352] - Detection of human TORC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TORC1 antibody NB100-2352 used for IP at 3 ug per reaction. TORC1 was also immunoprecipitated by another rabbit anti-TORC1 antibody. For blotting immunoprecipitated TORC1, NB100-2352 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TORC1 Antibody Summary

• Description: Novus Biologicals Rabbit TORC1 Antibody (NB100-2352) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 600 and the C-terminus (residue 634) of human Transducer of Regulated cAMP Response element-binding protein 1 using the numbering given in entry NP_056136.1 (GeneID 23373 ).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRTC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot
• Application Notes: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TORC1 Antibody

• CREB regulated transcription coactivator 1
• CRTC1
• FLJ14027WAMTP1
• KIAA0616Transducer of regulated cAMP response element-binding protein 1
• MECT1
• MECT1CREB-regulated transcription coactivator 1
• mucoepidermoid carcinoma translocated 1
• Mucoepidermoid carcinoma translocated protein 1
• TORC1
• TORC1TORC-1
• Transducer of CREB protein 1
• transducer of regulated cAMP response element-binding protein (CREB) 1
• WAMTP1

Background

MECT1 (also known as MucoEpidermoid Carcinoma Translocated 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1 does not appear to modulate CREB1 DNA-binding activity but enhances the interaction of CREB1 with TAF4/TAFII-130. MECT1 translocates with MAML2 (MasterMind-Like Protein 2) to yield a fusion oncogene: t(11;19) (q21;p13). This translocation occurs in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. The novel fusion product that results disrupts the Notch signaling pathway. The fusion protein consists of the N-terminus of MECT1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of MECT1 has been detected in a small number of mucoepidermoid carcinomas. Multiple isoforms have been reported for the MECT1 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRTC1
• Entrez:
  • 23373(Human)
  • 382056(Mouse)
• Uniprot:
  • NP_056136.1(Human)