Topoisomerase I Antibody - BSA Free

Western Blot: Topoisomerase I Antibody [NBP1-30482] - Detection of Human TOP1 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody NBP1-30482 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody used for WB at 0.1 ug/ml. Detection: chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: Topoisomerase I Antibody [NBP1-30482] - Detection of human TOP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TOP1 antibody NBP1-30482 (lot NBP1-30482-3) used for IP at 6 ug per reaction. TOP1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-30482-2). For blotting immunoprecipitated TOP1, NBP1-30482 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Topoisomerase I Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Topoisomerase I Antibody - BSA Free (NBP1-30482) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-Topoisomerase I Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 715 and 765 of human DNA topoisomerase I using the numbering given in entry NP_003277.1 (GeneID 7150).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TOP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Topoisomerase I Antibody - BSA Free

• DNA topoisomerase 1
• DNA topoisomerase I
• EC 5.6.2.1
• EC 5.99.1.2
• TOP1
• TOPI
• topoisomerase (DNA) I
• Topoisomerase I
• type I DNA topoisomerase

Background

Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Topoisomerase I Antibody (NBP1-30482)(3)

Publications using NBP1-30482

• Chatzinikolaou G, Stratigi K, Siametis A et al. XPF interacts with TOP2B for R-loop processing and DNA looping on actively transcribed genes Science advances 2023-11-10 [PMID: 37939182] (WB, Mouse)
• Chatzinikolaou G, Stratigi K, Agathangelou K ERCC1-XPF Interacts with Topoisomerase II beta to Facilitate the Repair of Activity-induced DNA Breaks bioRxiv 2020-01-01 (ICC/IF, WB, Mouse)
• Chatzidoukaki O, Stratigi K, Goulielmaki E Et al. R-loops trigger the release of cytoplasmic ssDNAs leading to chronic inflammation upon DNA damage Sci Adv 2021-11-19 [PMID: 34797720] (WB, Mouse)
  
  Details:
  Citation using the DyLight 550 version of this antibody.

FAQs for Topoisomerase I Antibody (NBP1-30482). (Showing 1 - 1 of 1 FAQs).

1. Will this antibody work for IHC for mice lung sections embedded in paraffin?
   • NBP1-30482 has not been validated for use in IHC so it is not guaranteed. NBP1-90365 is a Topoisomerase I antibody that has been validated for use in IHC on paraffin-embedded tissues. This is the antibody I would recommend.

Bioinformatics

• Gene Symbol: TOP1
• Entrez:
  • 7150(Human)
• Uniprot:
  • P11387(Human)