Topoisomerase I Antibody


Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody used for WB at 0.1 ug/ml. more
Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-30482] - Human stomach adenocarcinoma.
Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate from HeLa (50ug), 293T (50ug), Jurkat (50ug), NIH3T3 (50ug), and TCKM (50ug) cells.
Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, IP
1 mg/ml

Order Details

Topoisomerase I Antibody Summary

The immunogen for this product maps to a region between residue 715 and 765 of human DNA topoisomerase I using the numbering given in entry NP_003277.1 (GeneID 7150).
Predicted Species
Porcine (100%), Canine (100%), Rabbit (100%), Rhesus Macaque (100%), Bovine (100%), Primate (100%), Guinea Pig (100%), Equine (100%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate buffer, pH 7 to 8
0.09% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
91 kDa

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee, Medaka fish, Duckbill platypus, White-tufted-ear marmoset, Green monkey and Northern white-cheeked gibbon.

Alternate Names for Topoisomerase I Antibody

  • DNA topoisomerase 1
  • DNA topoisomerase I
  • EC
  • TOPI
  • topoisomerase (DNA) I
  • type I DNA topoisomerase


Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Topoisomerase I Antibody (NBP1-30482). (Showing 1 - 1 of 1 FAQ).

  1. Will this antibody work for IHC for mice lung sections embedded in paraffin?
    • NBP1-30482 has not been validated for use in IHC so it is not guaranteed. NBP1-90365 is a Topoisomerase I antibody that has been validated for use in IHC on paraffin-embedded tissues. This is the antibody I would recommend.

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol TOP1

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