Topoisomerase I Antibody

Images

 
Western Blot: Topoisomerase I Antibody [NBP1-30482] - Detection of Human TOP1 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. ...read more
Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate from HeLa (50ug), 293T (50ug), Jurkat (50ug), NIH3T3 (50ug), and TCKM (50ug) cells.
Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.
Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody used for WB at 0.1 ug/ml. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

Topoisomerase I Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 715 and 765 of human DNA topoisomerase I using the numbering given in entry NP_003277.1 (GeneID 7150).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TOP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2 - 10 ug/mg lysate
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Topoisomerase I Antibody

  • DNA topoisomerase 1
  • DNA topoisomerase I
  • EC 5.99.1.2
  • TOPI
  • topoisomerase (DNA) I
  • type I DNA topoisomerase

Background

Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Topoisomerase I Antibody (NBP1-30482). (Showing 1 - 1 of 1 FAQ).

  1. Will this antibody work for IHC for mice lung sections embedded in paraffin?
    • NBP1-30482 has not been validated for use in IHC so it is not guaranteed. NBP1-90365 is a Topoisomerase I antibody that has been validated for use in IHC on paraffin-embedded tissues. This is the antibody I would recommend.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TOP1
Entrez
Uniprot