TOM1L2 Antibody

Western Blot: TOM1L2 Antibody [NB300-991] - (0.03ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TOM1L2 Antibody [NB300-991] - Analysis in human Skeletal Muscle shows variable staining from fibre to fibre.

• Reactivity: Hu, Mu, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TOM1L2 Antibody Summary

• Description: Novus Biologicals Goat TOM1L2 Antibody (NB300-991) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-TOM1L2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CRKKPERSEDALFAL corresponding to C-Terminus according to NP_001028723.1, NP_001076437.1.
• Specificity: This antibody is expected to recognise both reported isoforms.
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TOM1L2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 1 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human fibroblast A431 lysates (calculated MW of 51.8 kDa band according to NP_653279). IHC-P: Human skeletal muscle shows variable staining from fibre to fibre.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TOM1L2 Antibody

• FLJ21501
• FLJ32746
• target of myb1 (chicken) homolog-like 1
• target of myb1-like 2 (chicken)
• Target of Myb-like protein 2
• TOM1-like protein 2

Background

Tom1L2 (Tom1-like2) together with Tom1L1 (Tom1-like1) and related proteins Tom1 (Target of Myb1) constitute a new protein family characterized by the presence of a VHS (Vps27p/Hrs/Stam) domain in the N-terminal portion followed by a GAT (GGA and Tom) domain. The molecular function of TOM1L2 is as an intra-Golgi protein transporter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOM1L2 Antibody (NB300-991)(1)

Publication using NB300-991

• Blagoev B, Ong SE, Kratchmarova I, Mann M. Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat Biotechnol 2004-09-01 [PMID: 15314609]

Bioinformatics

• Gene Symbol: TOM1L2
• Entrez:
  • 146691(Human)