TNF RII/TNFRSF1B Overexpression Lysate (Native)

Images

 
Western Blot: TNF Receptor II Overexpression Lysate (Adult Normal) [NBL1-17155] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNF Receptor II.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TNF RII/TNFRSF1B Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208641 and based on accession number NM_001066. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), mRNA.
Gene
TNFRSF1B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TNF RII/TNFRSF1B

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TNF RII/TNFRSF1B Overexpression Lysate (Native)

  • CD120b antigen
  • CD120b
  • Etanercept
  • p75 TNF receptor
  • p75TBPII
  • p75TNFR
  • soluble TNFR1B variant 1
  • TNF RII
  • TNFBRp80 TNF-alpha receptor
  • TNF-R2
  • TNFR2TNFR1B
  • TNF-R75
  • TNFR80
  • TNFRII
  • TNF-RII
  • TNF-R-II
  • TNFR-II
  • TNFRSF1B
  • tumor necrosis factor beta receptor
  • tumor necrosis factor binding protein 2
  • Tumor necrosis factor receptor 2
  • tumor necrosis factor receptor superfamily member 1B
  • tumor necrosis factor receptor superfamily, member 1B
  • Tumor necrosis factor receptor type II

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB367
Species: Hu, Ca
Applications: WB, IHC, ICC
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
H00005034-B01P
Species: Hu
Applications: WB, ICC/IF
MAB7745
Species: Hu
Applications: WB, Simple Western, IHC
MAB6638
Species: Hu, Mu, Rt
Applications: WB
NBP2-60655
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83227
Species: Hu, Mu(-)
Applications: WB, IHC, IHC-P
H00004354-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NBP1-87801
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF175
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NBL1-17155
Species: Hu
Applications: WB

Publications for TNF RII/TNFRSF1B Lysate (NBL1-17155) (0)

There are no publications for TNF RII/TNFRSF1B Lysate (NBL1-17155).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TNF RII/TNFRSF1B Lysate (NBL1-17155) (0)

There are no reviews for TNF RII/TNFRSF1B Lysate (NBL1-17155). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TNF RII/TNFRSF1B Lysate (NBL1-17155) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TNF RII/TNFRSF1B Products

Bioinformatics Tool for TNF RII/TNFRSF1B Lysate (NBL1-17155)

Discover related pathways, diseases and genes to TNF RII/TNFRSF1B Lysate (NBL1-17155). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TNF RII/TNFRSF1B Lysate (NBL1-17155)

Discover more about diseases related to TNF RII/TNFRSF1B Lysate (NBL1-17155).
 

Pathways for TNF RII/TNFRSF1B Lysate (NBL1-17155)

View related products by pathway.

PTMs for TNF RII/TNFRSF1B Lysate (NBL1-17155)

Learn more about PTMs related to TNF RII/TNFRSF1B Lysate (NBL1-17155).
 

Research Areas for TNF RII/TNFRSF1B Lysate (NBL1-17155)

Find related products by research area.

Blogs on TNF RII/TNFRSF1B

There are no specific blogs for TNF RII/TNFRSF1B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-2 [Unconjugated]
202-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TNF RII/TNFRSF1B Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFRSF1B
Entrez