Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

ELISA: Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric) [NBP2-60129] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric) [NBP2-60129] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 7.8125 pg/mL - 500 pg/mL
• Sensitivity: 1.6 pg/mL

Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse TNFsR- II . No significant cross-reactivity or interference between mouse TNFsR-II and analogues was observed.
• Standard Curve Range: 7.8125 pg/mL - 500 pg/mL
• Sensitivity: 1.6 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNFRSF1B

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

• CD120b antigen
• CD120b
• Etanercept
• p75 TNF receptor
• p75TBPII
• p75TNFR
• soluble TNFR1B variant 1
• TNF RII
• TNFBRp80 TNF-alpha receptor
• TNF-R2
• TNFR2TNFR1B
• TNF-R75
• TNFR80
• TNFRII
• TNF-RII
• TNF-R-II
• TNFR-II
• TNFRSF1B
• tumor necrosis factor beta receptor
• tumor necrosis factor binding protein 2
• Tumor necrosis factor receptor 2
• tumor necrosis factor receptor superfamily member 1B
• tumor necrosis factor receptor superfamily, member 1B
• Tumor necrosis factor receptor type II

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFsR- II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFsR-II present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFsR-II is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFsR-II bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF1B