Tmp21/p23 Antibody

Western Blot:[NBP3-30633]-Detection of human TMED10/TMP21 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, A-549, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TMED10/TMP21 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Tmp21/p23 Antibody Summary

• Description: Novus Biologicals Rabbit Tmp21/p23 Antibody (NBP3-30633) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 25 and 75
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMED10
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Tmp21/p23 Antibody

• 21 kDa transmembrane-trafficking protein
• p23
• P24(DELTA)
• p24delta
• S31I125
• S31III125
• TMED10
• Tmp21
• TMP21Tmp-21-I
• transmembrane emp24 domain-containing protein 10
• transmembrane emp24-like trafficking protein 10 (yeast)
• Transmembrane protein Tmp21,21 kDa transmembrane trafficking protein

Background

TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase, but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites, and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21, indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity and Abeta production, but not epsilon-secretase activity, demonstrating that TMP21 can modulate gamma-secretase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMED10