Tmp21/p23 Antibodies
We offer Tmp21/p23 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each Tmp21/p23 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Tmp21/p23 Antibodies can be used in a variety of model species: Bovine, Chicken, Human, Mouse, Primate, Rabbit, Rat, Xenopus. Use the list below to choose the Tmp21/p23 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Tmp21/p23 polyclonal antibodies.
Alternate Names for Tmp21/p23 Antibodies
anti-Tmp21/p23 antibody, anti-TMED10 antibody, anti-21 kDa transmembrane-trafficking protein antibody, anti-p23 antibody, anti-P24(DELTA) antibody, anti-p24delta antibody, anti-S31I125 antibody, anti-S31III125 antibody, anti-TMP21Tmp-21-I antibody, anti-transmembrane emp24 domain-containing protein 10 antibody, anti-transmembrane emp24-like trafficking protein 10 (yeast) antibody, anti-Transmembrane protein Tmp21,21 kDa transmembrane trafficking protein antibody
15 Results for "tmp21-p23" in Primary Antibodies
![Immunohistochemistry: Tmp21/p23 Antibody [NB110-57586] - TMP21 Antibody [NB110-57586] - Detection of TMP21 in central neurons.](http://images.novusbio.com/fullsize/TMP21-Antibody-Immunohistochemistry-NB110-57586-img0005.jpg "Immunohistochemistry: Tmp21/p23 Antibody [NB110-57586] - TMP21 Antibody [NB110-57586] - Detection of TMP21 in central neurons.")
![<b>Genetic Strategies Validation. </b>Western Blot: Tmp21/p23 Antibody [NB110-57586] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TMP21/TMED10 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human TMP21/TMED10 Polyclonal Antibody (Catalog # NB110-57586) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TMP21/TMED10 at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Knockdown-Validated-NB110-57586-img0006.jpg)
![Western Blot: Tmp21/p23 Antibody [NB110-57586] - TMP21 Antibody [NB110-57586] - Detection of TMP21 in human liver lysate.](http://images.novusbio.com/fullsize/TMP21-Antibody-Western-Blot-NB110-57586-img0004.jpg)
![Western Blot: Tmp21/p23 Antibody [NB110-57587] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TMP21/TMED10 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human TMP21/TMED10 Polyclonal Antibody (Catalog # NB110-57587) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TMP21/TMED10 at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reduc](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Knockdown-Validated-NB110-57587-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Tmp21/p23 Antibody [NB110-57587] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TMP21/TMED10 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human TMP21/TMED10 Polyclonal Antibody (Catalog # NB110-57587) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TMP21/TMED10 at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reduc")
![<b>Genetic Strategies Validation. </b>Western Blot: TMP21 Antibody [NB110-57587] - Detection of TMP21 in human liver lysate using NB110-57587.](http://images.novusbio.com/fullsize/TMP21-Antibody-Western-Blot-NB110-57587-img0003.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NB110-57587] - Tmp21/p23 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human TMP21/p23 polyclonal Antibody (Catalog # NB110-57587) at 1 ug/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NB110-57587-img0004.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-47600] - Analysis in human endometrium and skeletal muscle tissues using NBP2-47600 antibody. Corresponding TMED10 RNA-seq data are presented for the same tissues](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-47600-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-47600] - Analysis in human endometrium and skeletal muscle tissues using NBP2-47600 antibody. Corresponding TMED10 RNA-seq data are presented for the same tissues")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-47600] - Staining of human colon, kidney, pancreas and testis using Anti-TMED10 antibody NBP2-47600 (A) shows similar protein distribution across tissues to independent antibody NBP2-48969 (B).](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-47600-img0010.jpg)
![Western Blot: Tmp21/p23 Antibody [NBP2-47600] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Western-Blot-NBP2-47600-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Tmp21/p23 Antibody [NBP2-47600] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47600-img0006.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-47600] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-47600-img0016.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-47600] - Staining of humanendometrium shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-47600-img0013.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-47600] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-47600-img0014.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-47600] - Staining of human salivary gland shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-47600-img0015.jpg)
![Western Blot: Tmp21/p23 Antibody [NBP2-48969] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Western-Blot-NBP2-48969-img0004.jpg "Western Blot: Tmp21/p23 Antibody [NBP2-48969] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-48969] - Staining of human kidney.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-48969-img0013.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-48969] - Staining of human stomach shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-48969-img0005.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-48969] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-48969-img0006.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-48969] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-48969-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-48969] - Staining in human thyroid gland and skeletal muscle tissues using anti-TMED10 antibody. Corresponding TMED10 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-48969-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-48969] - Staining of human colon, kidney, pancreas and testis using Anti-TMED10 antibody NBP2-48969 (A) shows similar protein distribution across tissues to independent antibody NBP2-47600 (B).](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-48969-img0009.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-48969] - Staining of human testis.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-48969-img0010.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-48969] - Staining of human colon.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-48969-img0011.jpg)
![Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NBP2-48969] - Staining of human pancreas.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunohistochemistry-Paraffin-NBP2-48969-img0012.jpg)
![Western Blot: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Western blot analysis of Tmp21/p23 on different lysates with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: U-87 MG cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse pancreas tissue lysate (40 ug/Lane) Lane 9: Rat pancreas tissue lysate (40 ug/Lane) Lane 10: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 25 kDa Observed band size: 19 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33031) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33031_rabbit-tmp21-p23-mab-psh01-41-288202412493461.jpg "Western Blot: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Western blot analysis of Tmp21/p23 on different lysates with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: U-87 MG cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse pancreas tissue lysate (40 ug/Lane) Lane 9: Rat pancreas tissue lysate (40 ug/Lane) Lane 10: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 25 kDa Observed band size: 19 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33031) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Tmp21/p23 with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33031, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33031_rabbit-tmp21-p23-mab-psh01-41-298202414161859.jpg)
![Immunohistochemistry: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33031) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33031_rabbit-tmp21-p23-mab-psh01-41-298202413585570.jpg)
![Immunocytochemistry/ Immunofluorescence: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Immunocytochemistry analysis of HeLa cells labeling Tmp21/p23 with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Tmp21/p23 antibody (NBP3-33031) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33031_rabbit-tmp21-p23-mab-psh01-41-29202410234260.jpg)
![Flow Cytometry: Tmp21/p23 Antibody (PSH01-41) [NBP3-33031] - Flow cytometric analysis of HeLa cells labeling Tmp21/p23. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33031, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33031_rabbit-tmp21-p23-mab-psh01-41-29202416453210.jpg)
![Western Blot: Tmp21/p23 Antibody [NBP3-03691] - Analysis of extracts from normal (control) and TMED10 knockout (KO) 293T cells, using Tmp21/p23 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Knockout-Validated-NBP3-03691-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Tmp21/p23 Antibody [NBP3-03691] - Analysis of extracts from normal (control) and TMED10 knockout (KO) 293T cells, using Tmp21/p23 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Tmp21/p23 Antibody - Azide and BSA Free [Tmp21/p23] - Immunofluorescence analysis of NIH-3T3 cells using Tmp21/p23 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03691_rabbit-polyclonal-tmp21-p23-antibody-52202516293995.jpg)
![Immunohistochemistry: Tmp21/p23 Antibody - Azide and BSA Free [Tmp21/p23] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using Tmp21/p23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03691_rabbit-polyclonal-tmp21-p23-antibody-5220251636388.jpg)
![Immunohistochemistry: Tmp21/p23 Antibody - Azide and BSA Free [Tmp21/p23] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Tmp21/p23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03691_rabbit-polyclonal-tmp21-p23-antibody-52202516321464.jpg)
![Immunohistochemistry: Tmp21/p23 Antibody - Azide and BSA Free [Tmp21/p23] - Immunohistochemistry analysis of paraffin-embedded Human liver damage using Tmp21/p23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03691_rabbit-polyclonal-tmp21-p23-antibody-5220251639625.jpg)
![Western Blot: Tmp21/p23 Antibody - Azide and BSA Free [Tmp21/p23] - Western blot analysis of various lysates using Tmp21/p23 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-03691_rabbit-polyclonal-tmp21-p23-antibody-5220251640262.jpg)
![Western Blot: Tmp21/p23 Antibody (PSH01-40) [NBP3-33030] - Western blot analysis of Tmp21/p23 on different lysates with Rabbit anti-Tmp21/p23 antibody (NBP3-33030) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: Ramos cell lysate (20 ug/Lane) Lane 7: U-87 MG cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: Neuro-2a cell lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (20 ug/Lane) Lane 11: Mouse pancreas tissue lysate (40 ug/Lane) Lane 12: Mouse liver tissue lysate (40 ug/Lane) Lane 13: Rat pancreas tissue lysate (40 ug/Lane) Lane 14: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 25 kDa Observed band size: 19 kDa Exposure time: 2 minutes 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33030) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33030_rabbit-tmp21-p23-mab-psh01-40-288202412493433.jpg "Western Blot: Tmp21/p23 Antibody (PSH01-40) [NBP3-33030] - Western blot analysis of Tmp21/p23 on different lysates with Rabbit anti-Tmp21/p23 antibody (NBP3-33030) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: Raji cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: Ramos cell lysate (20 ug/Lane) Lane 7: U-87 MG cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: Neuro-2a cell lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (20 ug/Lane) Lane 11: Mouse pancreas tissue lysate (40 ug/Lane) Lane 12: Mouse liver tissue lysate (40 ug/Lane) Lane 13: Rat pancreas tissue lysate (40 ug/Lane) Lane 14: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 25 kDa Observed band size: 19 kDa Exposure time: 2 minutes 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33030) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tmp21/p23 Antibody (PSH01-40) [NBP3-33030] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Tmp21/p23 with Rabbit anti-Tmp21/p23 antibody (NBP3-33030) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33030, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33030_rabbit-tmp21-p23-mab-psh01-40-29820241435298.jpg)
![Immunohistochemistry: Tmp21/p23 Antibody (PSH01-40) [NBP3-33030] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Tmp21/p23 antibody (NBP3-33030) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33030) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33030_rabbit-tmp21-p23-mab-psh01-40-298202414161810.jpg)
![Immunocytochemistry/Immunofluorescence: Tmp21/p23 Antibody [NBP2-75965] - Paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75965-img0001.jpg "Immunocytochemistry/Immunofluorescence: Tmp21/p23 Antibody [NBP2-75965] - Paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: Tmp21/p23 Antibody [NBP2-75965] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Tmp21-p23-Antibody-Flow-Cytometry-NBP2-75965-img0002.jpg)
![Western Blot:[NBP3-29665]-Detection of human and mouse TMED10 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TMED10 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29665_rabbit-tmp21-p23-pab-231202512101642.jpg "Western Blot:[NBP3-29665]-Detection of human and mouse TMED10 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TMED10 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:Tmp21/p23 AntibodyNBP3-29543] -Detection of human TMED10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TMED10 antibody NBP3-29543 (lot NBP3-29543-1) used for IP at 6 µg per reaction. TMED10 was also immunoprecipitated by rabbit anti-TMED10.. For blotting immunoprecipitated TMED10, NBP3-29543 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29543_rabbit-tmp21-p23-pab-3012202416181814.jpg "Immunoprecipitation:Tmp21/p23 AntibodyNBP3-29543] -Detection of human TMED10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TMED10 antibody NBP3-29543 (lot NBP3-29543-1) used for IP at 6 µg per reaction. TMED10 was also immunoprecipitated by rabbit anti-TMED10.. For blotting immunoprecipitated TMED10, NBP3-29543 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")