Tmp21/p23 Antibody - BSA Free

Western Blot: Tmp21/p23 Antibody [NBP3-03691] - Analysis of extracts from normal (control) and TMED10 knockout (KO) 293T cells, using Tmp21/p23 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry/ Immunofluorescence: Tmp21/p23 Antibody - Azide and BSA Free [Tmp21/p23] - Immunofluorescence analysis of NIH-3T3 cells using Tmp21/p23 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry: Tmp21/p23 Antibody - Azide and BSA Free [Tmp21/p23] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using Tmp21/p23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry: Tmp21/p23 Antibody - Azide and BSA Free [Tmp21/p23] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Tmp21/p23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry: Tmp21/p23 Antibody - Azide and BSA Free [Tmp21/p23] - Immunohistochemistry analysis of paraffin-embedded Human liver damage using Tmp21/p23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western Blot: Tmp21/p23 Antibody - Azide and BSA Free [Tmp21/p23] - Western blot analysis of various lysates using Tmp21/p23 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Tmp21/p23 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-190 of human Tmp21/p23 (NP_006818.3). HLPINSRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMED10
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Knockout Validated
  • Western Blot 1:500-1:2000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Tmp21/p23 Antibody - BSA Free

• 21 kDa transmembrane-trafficking protein
• p23
• P24(DELTA)
• p24delta
• S31I125
• S31III125
• TMED10
• Tmp21
• TMP21Tmp-21-I
• transmembrane emp24 domain-containing protein 10
• transmembrane emp24-like trafficking protein 10 (yeast)
• Transmembrane protein Tmp21,21 kDa transmembrane trafficking protein

Background

TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase, but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites, and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21, indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity and Abeta production, but not epsilon-secretase activity, demonstrating that TMP21 can modulate gamma-secretase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: TMED10