TMEM26 Antibody - BSA Free

Western Blot: TMEM26 Antibody [NBP2-27334] - Total protein from Human Testis, Spleen, Colon, Kidney and Brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TMEM26 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Simple Western: TMEM26 Antibody [NBP2-27334] - Image shows a specific band for TMEM26 in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, Flow, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TMEM26 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TMEM26 Antibody - BSA Free (NBP2-27334) is a polyclonal antibody validated for use in IHC, WB, Flow and Simple Western. Anti-TMEM26 Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 300-350 of human Tmem26 was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMEM26
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry reported in scientific literature (PMID 30089732)
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 28250021)
  • Simple Western 1:10
  • Western Blot 1 - 2 ug/mL
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 27 kDa.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28844880).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for TMEM26 Antibody - BSA Free

• DKFZp686D09128
• Em:AC068892.1
• MGC35010
• transmembrane protein 26

Background

Transmembrane protein 26, or Tmem26, is a protein which spans the entire biological membrane and thus maintains three dimensional structures. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction; although some of them (beta-barrels) can be also extracted using denaturing agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TMEM26 Antibody (NBP2-27334)(11)

Publications using NBP2-27334

• Said Lhamyani, Adriana-Mariel Gentile, María Mengual-Mesa, Elia Grueso, Rosa M Giráldez-Pérez, José Carlos Fernandez-Garcia, Antonio Vega-Rioja, Mercedes Clemente-Postigo, John R Pearson, Isabel González-Mariscal, Gabriel Olveira, Francisco-Javier Bermudez-Silva, Rajaa El Bekay Au 16-pH-16/miR-21 mimic nanosystem: An efficient treatment for obesity through browning and thermogenesis induction. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024-01-09 [PMID: 38198956]
• Cheong LY, Wang B, Wang Q et al. Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice Nature communications 2023-03-03 [PMID: 36869026] (Flow Cytometry, Mouse)
• Lhamyani S, Gentile A, GirAldez-PErez R et al. miR-21 mimic blocks obesity in mice: a novel therapeutic option Mol Ther Nucleic Acids 2021-09-23 [PMID: 34552821]
• Finlin BS, Memetimin H, Zhu B et al. Pioglitazone does not synergize with mirabegron to increase beige fat or further improve glucose metabolism JCI insight 2021-02-11 [PMID: 33571166] (IF/IHC, Human)
• Jimenez T, Friedman T, Vadgama J et al. Nicotine Synergizes with High-Fat Diet to Induce an Anti-Inflammatory Microenvironment to Promote Breast Tumor Growth Mediators of Inflammation 2020-12-13 [PMID: 33414685] (WB, Mouse)
• Kim S, Park JW, Lee MG et al. Reversine promotes browning of white adipocytes by suppressing miR-133a J. Cell. Physiol. 2018-08-21 [PMID: 30132867] (WB, Mouse)
• Finlin BS, Memetimin H, Confides AL et al. Human adipose beiging in response to cold and mirabegron. JCI Insight 2018-08-09 [PMID: 30089732] (IF/IHC, Human)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Huang Z, Zhong L, Lee JTH et al. The FGF21-CCL11 Axis Mediates Beiging of White Adipose Tissues by Coupling Sympathetic Nervous System to Type 2 Immunity Cell Metab. 2017-08-23 [PMID: 28844880] (FLOW, Mouse)
• Finlin BS, Zhu B, Confides AL et al. Mast Cells Promote Seasonal White Adipose Beiging in Humans. Diabetes 2017-05-01 [PMID: 28250021] (IHC-P, Human)
• Liu PS, Lin YW, Lee B et al. Reducing RIP140 expression in macrophage alters ATM infiltration, facilitates white adipose tissue browning and prevents high fat diet-induced insulin resistance. Diabetes. 2014-06-26 [PMID: 24969109] (IHC-P, Mouse)
  
  Details:
  Figs S2-S4: White adipose tissue. TMEM26 was used as a marker of beige fat.
• Wu J, Bostr0m P, Sparks LM et al. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell. 2012-07-20 [PMID: 22796012]

Bioinformatics

• Gene Symbol: TMEM26
• Uniprot:
  • Q6ZUK4(Human)