TMEM119 Antibody (2699A) [Unconjugated]

TMEM119 was detected in immersion fixed paraffin-embedded sections of human lymph node using Rabbit Anti-Human TMEM119 Monoclonal Antibody (Catalog # MAB103131) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

HEK293 human embryonic kidney cell line transfected with (A) human TMEM119 or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Human TMEM119 Monoclonal Antibody (Catalog # MAB103131) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: 2699A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

TMEM119 Antibody (2699A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese Hamster Ovary cell line CHO derived human TREM119
  Arg26-Met96
  Accession # Q4V9L6.1
• Specificity: Detects human TREM119 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TMEM119 Antibody (2699A) [Unconjugated]

• OBIF
• Osteoblast Induction Factor
• TMEM119

Background

TMEM119 (Transmembrane Protein 119, also known as Osteoblast Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane protein that is predominantly expressed in osteoblasts and is upregulated during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimer's disease (3).  Mature human TMEM119 consists of a 71 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 166 aa cytoplasmic domain. Within the ECD, human TMEM119 shares 78% and 75% aa sequence identity with mouse and rat TMEM119, respectively. TMEM-119 is involved in the osteoblast differentiation and bone development by acting as a ligand and has been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (3, 4).

1. Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
2. Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
3. Satoh, J. et al. (2016) Neuropathol. 36:39.
4. Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

• Uniprot:
  • Q4V9L6.1()