TMED1 Overexpression Lysate (Native)

Images

 
Western Blot: TMED1 Overexpression Lysate (Adult Normal) [NBL1-16979] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMED1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TMED1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200255 and based on accession number NM_006858. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens transmembrane emp24 protein transport domain containing 1 (TMED1), mRNA.
Gene
TMED1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TMED1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TMED1 Overexpression Lysate (Native)

  • Il1rl1l
  • IL1RL1LGIL1RL1-binding protein
  • interleukin 1 receptor-like 1 ligand
  • Interleukin-1 receptor-like 1 ligand
  • MGC1270
  • Putative T1/ST2 receptor-binding protein
  • ST2L
  • T1/ST2 receptor binding protein
  • transmembrane emp24 domain containing 1
  • transmembrane emp24 domain-containing protein 1
  • transmembrane emp24 protein transport domain containing 1

Background

The protein encoded by this gene was identified by its interaction with interleukin 1 receptor-like 1 (IL1RL1). This protein lacks any similarity to other interleukin 1 ligands. The functional significance of its interaction with IL1RL1 is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF523
Species: Hu
Applications: WB, Simple Western, Flow, CyTOF-ready, Neut
AF3626
Species: Mu
Applications: WB, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
AF5828
Species: Hu
Applications: WB, ICC, Neut
NBP1-77148
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Flow-CS, Flow-IC
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
AF676
Species: Hu
Applications: WB, Flow, CyTOF-ready

Publications for TMED1 Lysate (NBL1-16979) (0)

There are no publications for TMED1 Lysate (NBL1-16979).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TMED1 Lysate (NBL1-16979) (0)

There are no reviews for TMED1 Lysate (NBL1-16979). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TMED1 Lysate (NBL1-16979) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TMED1 Products

Bioinformatics Tool for TMED1 Lysate (NBL1-16979)

Discover related pathways, diseases and genes to TMED1 Lysate (NBL1-16979). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Pathways for TMED1 Lysate (NBL1-16979)

View related products by pathway.

Blogs on TMED1

There are no specific blogs for TMED1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

TMED1 Antibody
NBP1-81595

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TMED1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol TMED1