TMED1 Antibody (1009524) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
1009524
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

TMED1 Antibody (1009524) [Alexa Fluor® 700] Summary

Immunogen
Human embryonic kidney cell, HEK293 derived human TMED1
Met1-Asn194
Accession # Q13445
Specificity
Detects human TMED1 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TMED1 Antibody (1009524) [Alexa Fluor® 700]

  • IL1RL1-Binding Protein
  • Il1rl1l
  • IL1RL1LG
  • IL-1RL1LG
  • IL1RL1LGIL1RL1-binding protein
  • Interleukin 1 Receptor-Like 1 Ligand
  • Interleukin-1 receptor-like 1 ligand
  • Ly84l
  • MGC1270
  • P24g1
  • P24gamma1
  • Putative T1/ST2 receptor-binding protein
  • ST2L
  • T1/ST2 receptor binding protein
  • TMED1
  • Tp24
  • transmembrane emp24 domain containing 1
  • transmembrane emp24 domain-containing protein 1
  • transmembrane emp24 protein transport domain containing 1

Background

TMED1 (Transmembrane Emp24 domain-containing protein 1) is a member of the TMED family of proteins (gene name TMED1). The TMED family of proteins are localized to membranes of the early secretory pathway, including the endoplasmic reticulum and Golgi, and function in vesicular protein trafficking (1, 2). TMED1 is a 59 kDa monomer and has been reported to exist as homodimer (3). TMED1 is composed of a 23 amino acid (aa) signal sequence, a 171 aa extra cellular domain, a 21 aa transmembrane domain, and a 12 aa cytoplasmic domain. The extracellular domain contains an 83 aa GOLD (Golgi Dynamics) domain, and COPI and COPII binding motifs are found in the cytoplasmic domain (1-3, 5). Human TMED1 shares 97% sequence identity with mouse, bovine, and rat homologs within the 171 aa extracellular domain. The beta -strand-rich GOLD domain has been specifically identified to be involved in intracellular protein trafficking (1, 4, 5). TMED1 is important in regulating innate immune signaling through its interaction with ST2L. Specifically, the GOLD domain in TMED1 interacts with the TIR domain of ST2L, a receptor for IL‑33 (1). This interaction promotes ST2L association with IL-33, allowing downstream signaling cascade activating MAP kinases, p38, and JNK (1, 6). Studies have shown knockdown of TMED-1 in HUVECs impairs the IL-33 induced response resulting in reduction of IL-6 and IL-8 productions (1).
  1. Connolly, D. et al. (2013) J Biol Chem. 288:5616.
  2. Gour, N. and Lajoie, S. (2018) Curr Allergy Ashma Rep. 16:65.
  3. Jenne, N. (2002) J Biol Chem. 277:46504.
  4. Anantharaman, V. and Aravind, L. (2002) Genome Biol. 3:research0023
  5. Gomez-Navarro, N. and Miller, E. (2016) J Cell Biol. 215:769.
  6. Hardman, C. and Ogg, G. (2016). Curr Opin Immunol. 42:16.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB22431U
Alexa Fluor 405 FAB22431V
Alexa Fluor 488 FAB22431G
Alexa Fluor 594 FAB22431T
Alexa Fluor 647 FAB22431R
Alexa Fluor 700 FAB22431N
Alexa Fluor 750 FAB22431S

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