TLE1 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

TLE1 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human TLE1
  His11-Asn200
  Accession # Q04724
• Specificity: Detects human TLE1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLE3 and rhTLE4 is observed and less than 5% cross-reactivity with rhTLE2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLE1 Antibody [Alexa Fluor® 350]

• E(Sp1) homolog
• enhancer of split groucho 1
• ESG
• ESG1Enhancer of split groucho-like protein 1
• GRG1
• TLE1
• transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
• transducin-like enhancer of split 1, homolog of Drosophila E(sp1)
• transducin-like enhancer protein 1

Background

TLE1 (Transducin-Like Enhancer of Split 1; also ESG1 and GRG1) is a member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed, and is known to antagonize NFkB and TCF (Wnt)-mediated signaling. TLE1 binds to other family members as a heterooligomer, or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of bound transcription factors. Human TLE1 is 770 amino acids (aa) in length. It contains a Gln‑rich region that mediates oligomerization (aa 1-131), a CCN domain that contains an NLS and CDC2 kinase site (aa 200-268), and six WD repeats that mediate protein‑protein interaction (aa 470-767). Phosphorylation on Ser will generate 90-93 kDa and 116 kDa forms in SDS‑Page. Potential splice variants exist. There are alternative start sites at Met57 and Met326, a 10 aa insertion after Gly124, and three distinct eight aa substitutions for aa 193-770, 193-199 and 445-770. Over aa 11-200, human TLE1 shares 98% aa identity with mouse TLE1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.