TIP120A Antibody - BSA Free

Western Blot: TIP120A Antibody [NBP1-49918] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TIP120A antibody NBP1-49918 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. TIP120A was also immunoprecipitated by rabbit anti-TIP120A antibody NBP1-49917, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry-Paraffin: TIP120A Antibody [NBP1-49918] - Section of human ovarian carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-CAND1 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Bv, Pm-Or
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TIP120A Antibody - BSA Free Summary

• Immunogen: The immunogen for this product maps to a region between residue 1180 and 1230 of human Cullin-Associated and Neddylation-Dissociated 1 using the numbering given in entry NP_060918.2 (GeneID 55832).
• Predicted Species: Rat (100%), Orangutan (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAND1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1:1000 - 1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TIP120A Antibody - BSA Free

• cullin-associated and neddylation-dissociated 1
• Cullin-associated and neddylation-dissociated protein 1
• cullin-associated NEDD8-dissociated protein 1
• DKFZp434M1414
• FLJ90441
• KIAA0829FLJ10114
• p120 CAND1
• TBP interacting protein
• TBP-interacting protein 120A
• TBP-interacting protein of 120 kDa A
• TIP120AFLJ38691
• TIP120FLJ10929

Background

CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIP120A Antibody (NBP1-49918)(2)

Publications using NBP1-49918

• Chagraoui J, Girard S, Spinella JF, et al. UM171 Preserves Epigenetic Marks that Are Reduced in Ex Vivo Culture of Human HSCs via Potentiation of the CLR3-KBTBD4 Complex Cell stem cell 2021-01-07 [PMID: 33417871]
• Sardina F, Pisciottani A, Ferrara M et al. Spastin recovery in hereditary spastic paraplegia by preventing neddylation-dependent degradation Life Sci Alliance 2020-12-01 [PMID: 33106322] (WB, IP, Human)

Bioinformatics

• Gene Symbol: CAND1